… a large number of essential genes for the bacterial pathogen, Staphylococcus aureus,
using a rapid shotgun antisense RNA method. Staphylococcus aureus chromosomal DNA …

… We have determined the first comprehensive list of S. aureus essential genes. This should act
as … is generic and could be applied to identify essential genes in other bacterial pathogens. …

… action is as an inhibitor of essential cellular targets and processes, we identify a set of 308
essential genes in the clinically important pathogen Staphylococcus aureus. A total of 446 …

… agents, we identified essential genes of Staphylococcus aureus N315 by using comparative
genomics … genes. In the present study, we found new essential genes in S aureus by using …

… the rapid screening for Staphylococcus aureus genes essential for cell viability and for the
introduction of specific polarity-neutral deletions in nonessential genes. The results obtained …

… average number of unique inserts per gene of 44.8. We identified LA-MRSA ST398 essential
genes comparable to other high-throughput S. aureus essential gene studies. As ST398 is …

… for determining essentiality of a gene during infection and for studying pathogenesis in this
organism. To titrate the expression level of expression of essential genes in vivo, we have …

… , thus providing potentially new essential gene targets for … IMPORTANCE Infections caused
by Staphylococcus aureus … on Antimicrobial Resistance in Staphylococcus aureus (NARSA) …

… gene could be lethal (Ludovice et al., 1998). In this study we showed that murE was indeed
an essential gene … To unambiguously determine whether the murE gene is an essential gene…

… aureus exposure. We identified several essential genes and pathways during the S.
aureus infection in our analysis. The results were further confirmed by western blot and qPCR …