Measurement of estradiol in human serum by LC-MS/MS using a novel estrogen-specific derivatization reagent

P Keski-Rahkonen, R Desai, M Jimenez… - Analytical …, 2015 - ACS Publications
… for the analysis of E2 in human serum by using a derivatization reagent with analyte-specific …
derivatization reagent 1,2-dimethylimidazole-5-sulfonyl chloride (DMIS). The derivatization

Highly sensitive determination of estrone and estradiol in human serum by liquid chromatography–electrospray ionization tandem mass spectrometry

K Yamashita, M Okuyama, Y Watanabe, S Honma… - Steroids, 2007 - Elsevier
… and estradiol in human serum was described based upon the use of picolinoyl derivatization
… / MS) [14] as reagents. Recently, the comparable detection sensitivity with the picolinoyl …

Sensitive routine liquid chromatography–tandem mass spectrometry method for serum estradiol and estrone without derivatization

S Pauwels, L Antonio, I Jans, A Lintermans… - Analytical and …, 2013 - Springer
… spectrometry method for estradiol and estrone in human serum without the need for
derivatization or extended extraction protocols. After protein precipitation of serum with a mixture of …

… of estradiol, estrone, and testosterone in postmenopausal human serum by isotope dilution liquid chromatography tandem mass spectrometry without derivatization

KM Wooding, JA Hankin, CA Johnson, JD Chosich… - Steroids, 2015 - Elsevier
serum without the use of derivatization reagents. In addition to the four steroids listed
above, we also analyzed P4 but excluded it from our final assay because of an unidentified, …

[HTML][HTML] Derivatization of estrogens enhances specificity and sensitivity of analysis of human plasma and serum by liquid chromatography tandem mass spectrometry

AMM Faqehi, DF Cobice, G Naredo, TCS Mak, R Upreti… - Talanta, 2016 - Elsevier
… groups suitable for targeting by derivatization reagents. Previous derivatization reagents
employed include dansyl chloride [11]… Estradiol and estrone were quantified in human serum or …

Development and evaluation of a reference measurement procedure for the determination of estradiol-17β in human serum using isotope-dilution liquid …

SSC Tai, MJ Welch - Analytical chemistry, 2005 - ACS Publications
… , to isolate estradiol from serum, and derivatization of estradiol with dansyl chloride 6,7 to …
, and all other chemicals were reagent grade. Frozen human serum samples from individual …

Measurement of human serum unconjugated estriol without derivatization using liquid chromatography-tandem mass spectrometry candidate reference method and …

X Huang, Q Zhang, S Zheng, J Wang, L Han… - Analytical and …, 2018 - Springer
… In addition, the different uE 3 derivatization products may show considerable variation in …
different uE 3 and derivatization reagent. Thus the preferred derivatization technique is often a …

Simultaneous determination of norethindrone and ethinyl estradiol in human plasma by high performance liquid chromatography with tandem mass spectrometry …

W Li, YH Li, AC Li, S Zhou, W Naidong - Journal of chromatography B, 2005 - Elsevier
estradiol, and their internal standards norethindrone- 13 C 2 , and ethinyl estradiol-d 4 , were
extracted from human plasma … solvent, the extract was derivatized with dansyl chloride and …

High-sensitivity micro LC-MS/MS assay for serum estradiol without derivatization

X Yi, EKY Leung, R Bridgman, S Koo… - Journal of Applied …, 2016 - academic.oup.com
… considerable demands to accurately measure estradiol (E 2 ) … preparations with dansyl
chloride derivatization. Our study … method without derivatization for E 2 in blood samples with …

Determination of estrogens in human serum using a novel chemical derivatization–assisted liquid chromatography‐electrospray ionization‐tandem mass spectrometry …

CX Kang, RT Fan, HM Xiao… - Rapid Communications in …, 2022 - Wiley Online Library
… To discover an appropriate derivatization reagent for estrogen … However, very limited
derivatization reagents for estrogen … appropriate derivatization reagent, we selected five reagents