Transfer and expression of foreign genes in mammalian cells

A Colosimo, KK Goncz, AR Holmes, K Kunzelmann… - …, 2000 - Taylor & Francis
… the parameters of mammalian expression vector systems and gene transfer methods. … to
deliver genes to and express genes in human cells. Lists of mammalian expression vectors can …

[HTML][HTML] Gene expression in mammalian cells and its applications

KH Khan - Advanced pharmaceutical bulletin, 2013 - ncbi.nlm.nih.gov
… , starting their career in gene expression related to mammalian cells. The principal result
and major … , expression system and applications of gene expression in mammalian cell lines. …

The production of foreign proteins in mammalian cells.

MM Bendig - Genetic Engineering (Academic Press), 1988 - europepmc.org
… transcription and translation of the foreign protein, higher expression levels can be achieved
by increasing the number of foreign gene copies per cell. High gene copy number is usually …

High-level expression of foreign genes in mammalian cells

SE Kane - Genetic engineering: principles and methods, 1991 - Springer
… Virtually all of the factors discussed in this article are dependent on the cell line to be
used for expressing foreign sequences (see ref. 10 for a review). While some studies can be …

Expression of foreign genes in mammalian cells

MJ Page - New Nucleic Acid Techniques, 1988 - Springer
… cloned gene into any cell type. It is not the purpose here to review all the expression constructs
… transient or long-term expression of gene products in mammalian cells, will be described. …

[39] Vectors used for expression in mammalian cells

RJ Kaufman - Methods in enzymology, 1990 - Elsevier
… and efficiently recognized in mammalian cells. There are many purposes for the use of
mammalian cells for expression of foreign genes: (1) To confirm that genes isolated by a variety of …

Targeting genes: delivery and persistent expression of a foreign gene driven by mammalian regulatory elements in vivo

CH Wu, JM Wilson, GY Wu - Journal of Biological Chemistry, 1989 - Elsevier
foreign gene driven by natural mammalian regulatory elements can be targeted to hepatocytes
and the resultant gene expression … Because the CAT gene is absent in mammalian cells, …

Overview of vector design for mammalian gene expression

RJ Kaufman - Molecular biotechnology, 2000 - Springer
… exhibit different rate limiting steps for efficient expression. Multiple … expression in mammalian
cells. This article reviews vector design for expression of foreign genes in mammalian cells. …

Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus

RJ Kaufman, MV Davies, LC Wasley… - Nucleic acids …, 1991 - academic.oup.com
… utility for the stable expression of foreign genes in mammalian cells. As shown here these
vectors are readily amplifiable to obtain high level expression of foreign genes. Introduction of …

Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors

I Shoji, H Aizaki, H Tani, K Ishii… - Journal of General …, 1997 - microbiologyresearch.org
… To express foreign genes in mammalian cells, we utilized the … of mammalian cell lines and
exhibits stronger expression … To examine expression of foreign genes in mammalian cells, …