Genome reduction boosts heterologous gene expression in Pseudomonas putida
S Lieder, PI Nikel, V de Lorenzo, R Takors - Microbial cell factories, 2015 - Springer
… Ongoing efforts include the adoption of Pseudomonas putida KT2440 variants with a
reduced genome as the functional chassis for biotechnological purposes. In these strains, …
reduced genome as the functional chassis for biotechnological purposes. In these strains, …
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression
… chemicals, the model soil bacterium Pseudomonas putida is naturally endowed with a number
of … The growing body of knowledge on P. putida strain KT2440 has been exploited for the …
of … The growing body of knowledge on P. putida strain KT2440 has been exploited for the …
Stochasticity of TOL plasmid catabolic promoters sets a bimodal expression regime in Pseudomonas putida mt‐2 exposed to m‐xylene
R Silva‐Rocha, V de Lorenzo - Molecular microbiology, 2012 - Wiley Online Library
The expression dynamics of the catabolic promoters of the TOL plasmid pWW0 has been
examined in single cells of Pseudomonas putida mt‐2 exposed to m‐xylene. To this end, we …
examined in single cells of Pseudomonas putida mt‐2 exposed to m‐xylene. To this end, we …
Transcriptomic fingerprinting of Pseudomonas putida under alternative physiological regimes
J Kim, JC Oliveros, PI Nikel… - Environmental …, 2013 - Wiley Online Library
… Pseudomonas putida KT2440 is a metabolically versatile soil bacterium useful both as a …
of various metabolic regimes into the genome-wide expression landscape of P. putida, we …
of various metabolic regimes into the genome-wide expression landscape of P. putida, we …
The pWW0 plasmid imposes a stochastic expression regime to the chromosomal ortho pathway for benzoate metabolism in Pseudomonas putida
R Silva-Rocha, V de Lorenzo - FEMS Microbiology Letters, 2014 - academic.oup.com
… P. putida strain alters also the single-cell expression regime of the recipient cells for catabolism
of benzoate and vice versa. Using a suite of transcriptional fusions to a green fluorescent …
of benzoate and vice versa. Using a suite of transcriptional fusions to a green fluorescent …
Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol
V Beckers, I Poblete-Castro, J Tomasch… - Microbial cell …, 2016 - Springer
… This study revealed the complex interplay of gene expression levels and metabolic fluxes
under … growth regimes on global gene expression and carbon flux distribution of pathways of P. …
under … growth regimes on global gene expression and carbon flux distribution of pathways of P. …
The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor
PI Nikel, FJ Romero-Campero, JA Zeidman… - MBio, 2015 - Am Soc Microbiol
… , we wondered whether the prolonged nongrowing regime of P. putida on glycerol was the
result of … KT2440 (which would determine the constitutive expression of the glp genes) and we …
result of … KT2440 (which would determine the constitutive expression of the glp genes) and we …
The IHF regulon of exponentially growing Pseudomonas putida cells
… putida strain and its ihfA derivative were determined by culturing cells to a steady-state
physiological regime with 13 C-labelled glucose. While expression of many transcripts was …
physiological regime with 13 C-labelled glucose. While expression of many transcripts was …
Spatial organization of the gene expression hardware in Pseudomonas putida
J Kim, A Goñi‐Moreno, B Calles… - Environmental …, 2019 - Wiley Online Library
… is independent of the biochemical regime of cells whether … to vary depending on gene
expression needs. Since the key … respect to the other two partners of the gene expression flow. …
expression needs. Since the key … respect to the other two partners of the gene expression flow. …
… synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression
… Pseudomonas putida KT2440 to use TA as sole carbon and energy source. A common aromatic
acid catabolite in P… hydrolase expression from P IbpA upon different heat shock regimes. …
acid catabolite in P… hydrolase expression from P IbpA upon different heat shock regimes. …
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