The impact of endogenous content, replicates and pooling on genome capture from faecal samples

J Hernandez‐Rodriguez, M Arandjelovic… - Molecular ecology …, 2018 - Wiley Online Library
captures significantly decrease the proportion of off-target reads from 34.15% after one
capture round to 7.83% after two capture … are advisable when using complex faecal samples. …

Whole mitochondrial genome capture from faecal samples and museum‐preserved specimens

T Van der Valk, F Lona Durazo, L Dalén… - Molecular ecology …, 2017 - Wiley Online Library
… in capturing complete mitochondrial genomes from faecal samples, but observe reduced
capture … here that mitochondrial genome capture from field-collected faecal samples is a robust …

[HTML][HTML] Whole genome sequences from non-invasively collected caribou faecal samples

RS Taylor, M Manseau, B Redquest… - Conservation Genetics …, 2021 - Springer
… We compare four faecal genomes to two tissue genomes … Quality metrics were similar between
faecal and tissue samples … the capture method still does not give unbiased whole genome

A roadmap for high‐throughput sequencing studies of wild animal populations using noninvasive samples and hybridization capture

LC White, C Fontsere, E Lizano… - Molecular Ecology …, 2019 - Wiley Online Library
… Specifically, after excluding the tissue-sample control libraries, we had 110 faecal sample
… that large-scale genomic studies are feasible for noninvasive samples through hybridization …

Maximizing the acquisition of unique reads in noninvasive capture sequencing experiments

C Fontsere, M Alvarez‐Estape, J Lester… - Molecular Ecology …, 2021 - Wiley Online Library
… of the whole genomic content of the samples, such as NGS, has … to capture the exome of 60
chimpanzee faecal samples as … targeted genomic regions may be increased in a collection of …

Genetic and genomic monitoring with minimally invasive sampling methods

EL Carroll, MW Bruford, JA DeWoody… - Evolutionary …, 2018 - Wiley Online Library
… RNA baits to capture genomic DNA from faecal samples from 62 … genomic markers to
undertake pedigree reconstruction (Snyder-Mackler et al., 2016). Another study, using bait captures

Urine as a high‐quality source of host genomic DNA from wild populations

AT Ozga, TH Webster, IC Gilby… - Molecular ecology …, 2021 - Wiley Online Library
… whole-genome capture MYBaits kit, we captured each sample according to the … faecal
samples to compare exome capture with whole-genome capture, and found that exome capture, …

[HTML][HTML] Methylation-based enrichment facilitates low-cost, noninvasive genomic scale sequencing of populations from feces

KL Chiou, CM Bergey - Scientific reports, 2018 - nature.com
… To expand the utility of the enrichment protocol to all fecal DNA samples, we conducted a
series of capture experiments designed to optimize the enrichment of host DNA from “low-…

Protocols for metagenomic DNA extraction and Illumina amplicon library preparation for faecal and swab samples

ATE Vo, JA Jedlicka - Molecular ecology resources, 2014 - Wiley Online Library
… used silica filter spin columns to bind and wash captured DNA. We aimed to improve upon
the … in bacterial 16S rRNA gene amplification success for faecal samples, the SPRI method …

[HTML][HTML] A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples

EM Batty, THN Wong, A Trebes, K Argoud, M Attar… - PloS one, 2013 - journals.plos.org
… C virus whole genome sequence … samples from faeces and two Hepatitis C virus samples
from blood, on an Illumina MiSeq benchtop sequencer. More than 97% of reference genomes