Time-domain fluorescence lifetime imaging applied to biological tissue

D Elson, J Requejo-Isidro, I Munro, F Reavell… - Photochemical & …, 2004 - Springer
… in fluorescence intensity across the image, we conclude that the observed variation in
fluorescence lifetime … 3(b) is not likely to be significant but the lifetime difference observed at the ‘…

Non-linear fluorescence lifetime imaging of biological tissues

R Cicchi, F Saverio Pavone - Analytical and bioanalytical chemistry, 2011 - Springer
… still to be done, including the interpretation of fluorescence lifetime data, we believe that this
… by using fluorescence lifetime imaging microscopy for the investigation of biological tissues. …

Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
… than elastin, as illustrated in Figure 29, where the mucosal surface composed of elastin
shows an excellent contrast in fluorescence lifetime with collagen rich tissue. In addition to …

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

J Siegel, DS Elson, SED Webb, KCB Lee, A Vlandas… - Applied …, 2003 - opg.optica.org
… study of biological tissue and … fluorescence lifetime data from real tissues, we present in
vitro microscopy studies of the complex fluorescence decay profiles observed in biological tissue

Multi‐wavelength fluorescence lifetime spectroscopy: a new approach to the study of endogenous fluorescence in living cells and tissues

D Chorvat Jr, A Chorvatova - Laser Physics Letters, 2009 - Wiley Online Library
… can serve to demarcate specific cell types in tissues, or organs, as well as their changes
in … between fluorescence lifetimes or spectra of endogenous fluorophores and biological

Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues

M Saito Nogueira, A Cosci… - Journal of …, 2017 - spiedigitallibrary.org
… performing fluorescence spectroscopy and fluorescence lifetime measurements in clinics,
its characterization, and its validation with measurements in biological tissues. Normal tissue, …

Fluorescence lifetime techniques in medical applications

L Marcu - Annals of biomedical engineering, 2012 - Springer
… of time-resolved (lifetime) fluorescence techniques used in … fluorescence spectroscopy
(TRFS) and fluorescence lifetime … and diagnosis of biological tissues. Emphasis is placed on the …

Fluorescence lifetime imaging of unstained tissues: early results in human breast cancer

PJ Tadrous, J Siegel, PMW French… - The Journal of …, 2003 - Wiley Online Library
… Although the basis of lifetime contrast in autofluorescence of tissue is not … fluorescence
and lifetimes. These include the pyridine nucleotides, where free NADH has a different lifetime

Methods for imaging the structure and function of living tissues and cells: 2. Fluorescence lifetime imaging

PJ Tadrous - The Journal of pathology, 2000 - Wiley Online Library
… The passage of excitation and fluorescent light through multiply scattering or heterogeneous
media (such as turbid biological tissues) can be described by the diffusion approximation to …

NAD (P) H fluorescence lifetime measurements in fixed biological tissues

JV Chacko, KW Eliceiri - … and Applications in Fluorescence, 2019 - iopscience.iop.org
… We do observe a change in the fluorescence lifetime and fluorescence spectral signature
with fixation, but the relative changes between any two samples in the NAD(P)H lifetime