Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
… to estimating fluorescence lifetimes from low SNR images. One implementation of global …
used to analyze hyperspectral fluorescence lifetime images and to dynamically monitor …

High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications

S Shrestha, BE Applegate, J Park, X Xiao, P Pande… - Optics Letters, 2010 - opg.optica.org
Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for characterizing
the biochemical composition of biological tissue. In recent years, there has been an …

Fluorescence lifetime imaging microscopy

CW Chang, D Sud, MA Mycek - Methods in cell biology, 2007 - Elsevier
… into wide‐field, confocal, and multiphoton systems, on each of which lifetime imaging can
be implemented. Wide‐field microscopy illuminates the entire specimen to provide single‐shot …

Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
… to the fluorescence intensity, fluorescence lifetime is a property of the fluorescent molecules
… Consequently, fluorescence lifetime imaging microscopy (FLIM) is nearly independent of the …

A practical implementation of multifrequency widefield frequency‐domain fluorescence lifetime imaging microscopy

H Chen, E Gratton - Microscopy research and technique, 2013 - Wiley Online Library
fluorescence lifetime imaging microscopy (FD-FLIM) is a fast and accurate method to measure
the fluorescence lifetime… Herein, we describe a practical implementation of multifrequency …

Fluorescence lifetime imaging microscopy (FLIM)

EB van Munster, TWJ Gadella - Microscopy Techniques: -/-, 2005 - Springer
… excited state lifetimes within microscopic images. FLIM systems have been implemented both
… In addition to technical implementation, this chapter gives an overview of the application of …

Hardware implementation algorithm and error analysis of high-speed fluorescence lifetime sensing systems using center-of-mass method

DU Li, B Rae, R Andrews, J Arlt… - Journal of biomedical …, 2010 - spiedigitallibrary.org
fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed
to implement lifetime … , general purpose fluorescence lifetime imaging microscopy (FLIM) …

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

S Cheng, RM Cuenca, B Liu, BH Malik… - Biomedical optics …, 2014 - opg.optica.org
… in the application of fluorescence lifetime imaging (FLIM) for … , capable of continuous lifetime
imaging at multiple emission … Alternatively, the fluorescence lifetime can be implemented

Generalization of the polar representation in time domain fluorescence lifetime imaging microscopy for biological applications: practical implementation

A Leray, C Spriet, D Trinel, Y Usson… - Journal of …, 2012 - Wiley Online Library
fluorescence lifetime images is increasingly used in time domain fluorescence lifetime imaging
… The calculations of the polar coordinates in time domain fluorescence lifetime imaging

A novel fluorescence lifetime imaging system that optimizes photon efficiency

RA Colyer, C Lee, E Gratton - Microscopy research and …, 2008 - Wiley Online Library
implementation for a low cost digital frequency domain FLIM (DFD-FLIM) system, which can
provide lifetime … Our implementation provides data natively in the form of phasors. On the …