[HTML][HTML] Engineered HaloTag variants for fluorescence lifetime multiplexing

MS Frei, M Tarnawski, MJ Roberti, B Koch, J Hiblot… - Nature …, 2022 - nature.com
… for further multiplexing 1,2,3,4,5 . To facilitate the use of fluorescence lifetime multiplexing
in … but become distinguishable based on their fluorescence lifetime. To achieve this, we …

Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes

MS Frei, B Koch, J Hiblot, K Johnsson - ACS Chemical Biology, 2022 - ACS Publications
fluorescence microscopy, few of them have been characterized for their use in fluorescence
lifetime multiplexing. … are suitable for fluorescence lifetime multiplexing in living mammalian …

Fluorescence lifetime multiplexing with nanocrystals and organic labels

M Grabolle, P Kapusta, T Nann, X Shu… - Analytical …, 2009 - ACS Publications
… in fluorescence lifetime that may … lifetime multiplexing with mixtures of QDs with spectrally
matching short-lived organic dyes and QDs. Advantageous for multicolor lifetime multiplexing

Tunable lifetime multiplexing using luminescent nanocrystals

Y Lu, J Zhao, R Zhang, Y Liu, D Liu, EM Goldys… - Nature …, 2014 - nature.com
… dimensions, such as mass spectra 15 , fluorescence lifetime 23 and Raman spectra 24 . These
… a new multiplexing concept by manipulating the luminescence decay lifetimes to create a …

Molecular probes for fluorescence lifetime imaging

P Sarder, D Maji, S Achilefu - Bioconjugate chemistry, 2015 - ACS Publications
… of intracellular or extracellular microenvironments, fluorescence lifetime (FLT) imaging has
… -responsive FLTs, information multiplexing can be readily accomplished without the need …

Development of a time domain fluorimeter for fluorescent lifetime multiplexing analysis

CD Salthouse, R Weissleder… - IEEE transactions on …, 2008 - ieeexplore.ieee.org
… on fluorescent lifetime. A mathematical framework for fluorescent lifetime multiplexing has
… in this work we are demonstrating that fluorescent lifetime multiplexing can be performed on …

[HTML][HTML] Fluorescence lifetime multiplexing with fluorogen activating protein FAST variants

YA Bogdanova, ID Solovyev, NS Baleeva… - Communications …, 2024 - nature.com
… genetically encoded system for multiplexed fluorescence lifetime microscopy based on the
… pairs exhibiting well-distinguishable fluorescence lifetimes. These chemogenetic probes, …

Chemical Control of Fluorescence Lifetime towards Multiplexing Imaging

J Ma, F Luo, CH Hsiung, J Dai, Z Tan… - Angewandte Chemie …, 2024 - Wiley Online Library
… , fluorescence lifetime multiplexing … in fluorescence lifetimes. Recently, Johnsson and
coworkers developed HaloTag variants that conjugate with Rhodamine to produce varying lifetimes

Preclinical whole body time domain fluorescence lifetime multiplexing of fluorescent proteins

WL Rice, ATN Kumar - Journal of biomedical optics, 2014 - spiedigitallibrary.org
… The application of time domain (TD) fluorescence lifetime multiplexing for the detection of
fluorescent proteins (FPs) in whole animals, in the presence of a strong background tissue …

Lifetime-based tomographic multiplexing

SB Raymond, DA Boas, BJ Bacskai… - Journal of biomedical …, 2010 - spiedigitallibrary.org
… We describe in vivo tomography of three spectrally overlapping fluorophores using
fluorescence lifetime-based separation. Time-domain images are acquired using a voltage-gated, …