Green fluorescent protein as a marker for Pseudomonas spp

GV Bloemberg, GA O'Toole… - Applied and …, 1997 - Am Soc Microbiol
… stably maintained in Pseudomonas spp. We … of Pseudomonas aeruginosa and Burkholderia
cepacia attached to an abiotic surface and (ii) the association of Pseudomonas fluorescens

Use of green fluorescent protein‐based reporters to monitor balanced production of antifungal compounds in the biocontrol agent Pseudomonas fluorescens CHA0

E Baehler, M Bottiglieri, M Péchy‐Tarr… - Journal of Applied …, 2005 - academic.oup.com
… the green fluorescent protein (… Pseudomonas fluorescens strain CHA0 to control plant
diseases caused by soil‐borne pathogenic fungi. Methods and Results: Pseudomonas fluorescens

Reliable use of green fluorescent protein in fluorescent pseudomonads

TM Timms-Wilson, MJ Bailey - Journal of microbiological methods, 2001 - Elsevier
… Here, we describe a simple but effective way of identifying gfp-expressing Pseudomonas
fluorescens using media supplemented with 0.45 mM FeSO 4 ·7H 2 O. This is of relevance for …

Green Fluorescent Protein-Marked Pseudomonas fluorescens: Localization, Viability, and Activity in the Natural Barley Rhizosphere

B Normander, NB Hendriksen… - Applied and …, 1999 - Am Soc Microbiol
… Information on the viability of the inoculant was provided by a microcolony assay, while
measurements of cell volume, the intensity of green fluorescent protein fluorescence, and the …

Effect of Starvation and the Viable-but-Nonculturable State on Green Fluorescent Protein (GFP) Fluorescence in GFP-TaggedPseudomonas fluorescens A506

M Lowder, A Unge, N Maraha, JK Jansson… - Applied and …, 2000 - Am Soc Microbiol
… of GFP fluorescent VBNC cells or if dead cells are fluorescent and … strain of Pseudomonas
fluorescens is fluorescent during … Also, we wanted to determine whether GFP fluorescence of …

Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria

R Tombolini, A Unge, ME Davey… - FEMS Microbiology …, 1997 - academic.oup.com
… In 1994, the gfp gene encoding the green fluorescent protein (GFP) from the jellyfish Aequorea
victoria was shown to be expressed in both eukaryotic and prokaryotic cells [6]. GFP is a …

A comparison of enumeration methods for culturable Pseudomonas fluorescens cells marked with green fluorescent protein

MB Cassidy, KT Leung, H Lee, JT Trevors - Journal of microbiological …, 2000 - Elsevier
… In this study, we used a gfp-tagged rhizosphere-colonizing Pseudomonas fluorescens strain
to investigate enumeration methods for culturable cells by determining survival in bulk soil, …

… of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein

Y Park, Y Moon, J Ryoo, N Kim, H Cho, JH Ahn - Microbial Cell Factories, 2012 - Springer
… type 1 secretion system (T1SS) of Pseudomonas fluorescens. The secretion is promoted by
its … fluorescent protein (GFP), and the secretion of EGF-LARD or GFP-LARD fusion proteins

Monitoring physiological status of GFP-tagged Pseudomonas fluorescens SBW25 under different nutrient conditions and in soil by flow cytometry

N Maraha, A Backman… - FEMS microbiology …, 2004 - academic.oup.com
… a dual gfp/lux AB marker system, encoding the green fluorescent protein and bacterial
luciferase, and the resultant strain was designated P. fluorescens SBW25::gfp/lux [44]. This strain …

Monitoring population size, activity, and distribution of gfp-luxAB-tagged Pseudomonas fluorescens SBW25 during colonization of wheat

A Unge, J Jansson - Microbial ecology, 2001 - Springer
fluorescens SBW25 to colonize seeds is of interest since the seed is often a major entry …
of the biocontrol agent P. chlororaphis on barley seeds using the green fluorescent protein (GFP) …