… Further omics approaches for the earliest β-cells in our MIP-Timer mice will lead to a better
understanding of the molecular mechanisms underlying β-cell neogenesis and maturation. …

… is preferentially secreted from β-cells. Here, using a novel fluorescent timer protein, we …
by variation in the β-cell environment, such as hyperglycemia. We demonstrate the use of a …

… , termed ‘Fluorescent Timer’, which shifts its fluorescence from … , green fluorescent cells without
red fluorescence were … GFP; Timer mice, whereas only green/red double-fluorescent cells …

… cell suspensions from both isolated islets and whole pancreas from MIP-GFP-transgenic mice
and sorted the β-cells by fluorescence-activated cell … -time instant identification of β-cells is …

… old) are secreted first from human pancreatic β-cells [9]. Because the methods using
fluorescent proteins have the limitation of their own fluorescence time, we used the HaloTag™ …

… -resolved analysis for endocrine differentiation, we previously developed reporter mouse
models that express DsRed-E5, called ‘fluorescent timer’, which shifts its fluorescence from …

… fluorescent protein DsRed-E5, called “Fluorescent Timer”, which shifts its fluorescence from
green to red over time [26], and developed the transgenic mouse model “Neurog3-Timer” in …

… The insulin-producing cells (ie, the β cells) already lost at the time of the clinical … fluorescence
in the β cells is more abundant. As time passes after synthesis, more of the fluorescence …

… (Ngn3-Timer) in which DsRed-E5, a fluorescent protein that … , fluorescent cells were
sorted by a fluorescence-activated cell … at various time points following culture. Green-dominant …

… of β cells in a time-dependent manner, the cells of … MIP-Timer pancreata were sorted by
FACS into three populations … Further OMICS approaches for the earliest β cells in our MIP-Timer …