… a forward genetic screen to identify components of the XopJ4 perception pathway in the model
plant N. benthamiana… benthamiana, which is a widely used model plant but is challenging …

… forward genetic screens using gene silencing have been performed in N. benthamiana, …
forward and reverse genetic analyses for this much-cherished model plant system (Fig. 1). …

… Analyses in Nicotiana species have shown that the symptoms on these plants arc … is referred
to as reverse genetics. VIGS can also be used in a forward genetics strate,gy although the …

… genes in Nicotiana benthamiana. In this manuscript, we demonstrate a forward genetics
screening … benthamiana and assessing the response of gene silenced plants for compromised …

… Nicotiana benthamiana has emerged as a complementary experimental system to Arabidopsis
thaliana. It enables fast-forward … benthamiana. We showed that ZAR1 stands out among …

… Here we report on a simple and reproducible system of Agrobacterium-mediated transient
gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. …

… tabacum is a tetraploid, it is difficult to obtain mutants in this species using forward genetic
approaches. Therefore, we used our TRV based VIGS approach to suppress the expression of …

… Virus-induced gene silencing (VIGS) is an efficient tool for high throughput reverse genetic
… Here we describe a protocol for conducting a VIGS screen in Nicotiana benthamiana using …

… The newly developed selection markers extend the applications of RGNs as directed
forward genetics tools via the analysis of transgene-free T 1 /T 2 segregants for a phenotype of …

… In addition, cDNA libraries have been developed in the TRV vector 38,39 for forward genetics
and large-scale silencing to identify plant genes that have a role during plant development …