FRET analysis reveals distinct conformations of IN tetramers in the presence of viral DNA or LEDGF/p75
JJ Kessl, M Li, M Ignatov, N Shkriabai… - Nucleic acids …, 2011 - academic.oup.com
… Our FRET studies show that IN tetramers formed in IN–viral DNA and IN–LEDGF/p75
complexes are distinct. The order of addition experiments ( Figure 6 ) further underscore the …
complexes are distinct. The order of addition experiments ( Figure 6 ) further underscore the …
Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-FRET
… FRET that overcome the complications in conventional FRET, … In this study, we demonstrate
that QD-FRET, with the energy … and composition of polymeric DNA nanocomplexes, as well …
that QD-FRET, with the energy … and composition of polymeric DNA nanocomplexes, as well …
A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses
L Ferreira da Silva, A Valle Garay, PF Queiroz… - PloS one, 2024 - journals.plos.org
… This method combines specific viral gene analysis, trans-… (FRET)-based hybridization of
fluorescent DNA hairpins. In this … ) with DNA hairpins containing fluorophores, leading to a FRET …
fluorescent DNA hairpins. In this … ) with DNA hairpins containing fluorophores, leading to a FRET …
FRET analysis of HIV‐1 Gag and GagPol interactions
S Takagi, F Momose, Y Morikawa - FEBS Open Bio, 2017 - Wiley Online Library
… (B) Virus particle production. HeLa cells were cotransfected with the Gag-expressing and
the GagPol-expressing molecular clones at a DNA ratio of 10 : 1. The particle fractions purified …
the GagPol-expressing molecular clones at a DNA ratio of 10 : 1. The particle fractions purified …
Detection of type and subtypes of influenza virus by hybrid formation of FRET probe with amplified target DNA and melting temperature analysis
SK Poddar - Journal of virological methods, 2003 - Elsevier
… In conclusion, the study verified the applicability of selected three sets of LightCycler FRET
probes for detection of influenza virus type and subtype specific gene fragments. Detection …
probes for detection of influenza virus type and subtype specific gene fragments. Detection …
Single-molecule and FRET fluorescence correlation spectroscopy analyses of phage DNA packaging: colocalization of packaged phage T4 DNA ends within the …
K Ray, J Ma, M Oram, JR Lakowicz, LW Black - Journal of molecular biology, 2010 - Elsevier
… In this work, we demonstrate by FRET-FCS and sm-FRET that the two ends of the DNA
packaged by the phage T4 motor are held in proximity in the procapsid. Similar results for 5-kbp …
packaged by the phage T4 motor are held in proximity in the procapsid. Similar results for 5-kbp …
Single-molecule FRET for virology: 20 years of insight into protein structure and dynamics
… DNA targets. Immobilised dsDNA constructs that featured a FRET pair exhibiting very close
proximity and high-FRET … The distinct FRET efficiencies showed that RT binds DNA and RNA …
proximity and high-FRET … The distinct FRET efficiencies showed that RT binds DNA and RNA …
Rapid detection and quantitation of hepatitis B virus DNA by real-time PCR using a new fluorescent (FRET) detection system
… and a viral encoded DNA polymerase. The genome contains four overlapping open reading
frames that encode for the viral DNA … targeting viral antigens and antibodies to viral proteins. …
frames that encode for the viral DNA … targeting viral antigens and antibodies to viral proteins. …
[HTML][HTML] Quantitative assays for maedi-visna virus genetic sequences and mRNA's based on RT-PCR with real-time FRET measurements
B Gudmundsson, H Bjarnadottir, S Kristjansdottir… - Virology, 2003 - Elsevier
… DNA and RNA molecules present at various stages of viral replication. Assay design was
based on PCR with real-time fluorescence resonance energy transfer (FRET… on target DNA (Fig…
based on PCR with real-time fluorescence resonance energy transfer (FRET… on target DNA (Fig…
Single-molecule FRET studies of HIV TAR–DNA hairpin unfolding dynamics
… In this article, we carried out single-molecule FRET experiments to study the complex dynamics
of HIV TAR–DNA … Further analysis of the distribution of the FRET efficiencies of the two …
of HIV TAR–DNA … Further analysis of the distribution of the FRET efficiencies of the two …
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