Analysis of transcripts and splice isoforms in Medicago sativa L. by single-molecule long-read sequencing

Y Chao, J Yuan, T Guo, L Xu, Z Mu, L Han - Plant Molecular Biology, 2019 - Springer
… including 46,616 full-length ORFs, 1670 transcription factors from … long non-coding RNAs.
Our results show the feasibility of deep sequencing full-length RNA from alfalfa transcriptome

Full-length transcriptome of in Medicago sativa L. roots in response to drought stress

Z Fang, J Liu, X Wu, Y Zhang, H Jia, Y Shi - Frontiers in Genetics, 2023 - frontiersin.org
… utilized the SMRT sequencing to generate and identify full-length transcriptome of Alfalfa roots
… the transcriptome data, we analyzed the full-length transcriptome sequences, transcription

Full-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity …

D Luo, Q Zhou, Y Wu, X Chai, W Liu, Y Wang, Q Yang… - BMC Plant …, 2019 - Springer
… In this study, we constructed the first full-length transcriptome database for alfalfa root tips …
-Seq. This resulted in the identification of 52,787 full-length transcripts, with an average length

Small RNA deep sequencing identifies novel and salt‐stress‐regulated microRNAs from roots of Medicago sativa and Medicago truncatula

RC Long, MN Li, JM Kang, TJ Zhang… - Physiologia …, 2015 - Wiley Online Library
Medicago, we used a high-throughput sequencing approach to analyze four small RNA
libraries from roots of Zhongmu-1 (Medicago sativa) and Jemalong A17 (Medicagotranscription

Comparative transcriptome investigation of global gene expression changes caused by miR156 overexpression in Medicago sativa

R Gao, RS Austin, L Amyot, A Hannoufa - BMC genomics, 2016 - Springer
Medicago sativa transcriptome using the Trinity program. a Summary of de novo assembly of
M. sativa transcriptome. … Transcript sequences of the aforementioned genes were obtained …

Development and characterization of simple sequence repeat (SSR) markers based on RNA-sequencing of Medicago sativa and in silico mapping onto the M …

Z Wang, G Yu, B Shi, X Wang, H Qiang, H Gao - PLoS One, 2014 - journals.plos.org
… The longer the SSR repeats were, the higher the rate of polymorphism in transcripts was …
repeats had sequence length in class I, accounting for 71.6% (3,216 SSRs) of total SSRs, …

Deciphering the regulatory network of miR156 in plant architecture and abiotic stress resistance of alfalfa (Medicago sativa) by transcriptome sequencing

H Zhang, S Jia, M Zhang, K Wang, F Teng, Y Liu… - Industrial Crops and …, 2022 - Elsevier
… with both 5′ and 3′ ends after removing the redundant sequences. A comparative analysis
of our unique full-length transcripts identified 12,596 … sativa cultivar "Zhongmu No.1". A …

Pan-transcriptome identifying master genes and regulation network in response to drought and salt stresses in Alfalfa (Medicago sativa L.)

CA Medina, DA Samac, LX Yu - Scientific reports, 2021 - nature.com
… of transcripts reconstruction based on short read sequences … -Seq to generate the full-length
transcripts and RNA-seq to correct the transcript sequences allowed us to identify full length

The Medicago sativa gene index 1.2: a web-accessible gene expression atlas for investigating expression differences between Medicago sativa subspecies

JA O'Rourke, F Fu, B Bucciarelli, SS Yang, DA Samac… - BMC genomics, 2015 - Springer
Transcripts from roots, nitrogen-fixing root nodules, leaves, flowers, elongating stem … the
Medicago sativa Gene Index 1.2 (MSGI 1.2) representing 112,626 unique transcript sequences. …

De Novo Characterization of Fall Dormant and Nondormant Alfalfa (Medicago sativa L.) Leaf Transcriptome and Identification of Candidate Genes Related to Fall …

S Zhang, Y Shi, N Cheng, H Du, W Fan, C Wang - PLoS One, 2015 - journals.plos.org
… Although the genome of alfalfa is unavailable at present, the genome size was predicted to
be 800 Mb[24]. Alfalfa leaf transcriptome sequencing in our study resulted in a relatively high …