Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase …

A Ramadan, K Nemoto, M Seki, K Shinozaki… - BMC plant biology, 2015 - Springer
… -containing proteins [14]. To construct a protein library of Arabidopsis RING proteins, we
collected 274 cDNA clones from the RAFL library [21] according to the annotated RING proteins […

Immunoassays: biological tools for high throughput screening and characterisation of combinatorial libraries

MÂ Taipa - Combinatorial Chemistry & High Throughput …, 2008 - ingentaconnect.com
… [6] screening and characterisation of lead ligands of combinatorial libraries from both biological
… tool for the functional characterisation of novel proteins emerging from proteomics work. …

Genetic code evolution investigated through the synthesis and characterisation of proteins from reduced‐alphabet libraries

MS Newton, DJ Morrone, KH Lee, B Seelig - ChemBioChem, 2019 - Wiley Online Library
… code,19 we defined three primordial protein alphabets that comprise the likely earliest five,
… synthesised protein libraries of random sequence—each 83 amino acids in length. Proteins

Structural Characterisation of Human Kinases using a Library-Based Construct Screening Approach

H Yumerefendi - 2009 - theses.hal.science
… soluble over-expression and structural characterisation. First, improvements in … libraries
leading to better coverage of the diversity. Then, its application on a more characterised protein, …

Functional information in SWISS-PROT: the basis for large-scale characterisation of protein sequences

R Apweiler - Briefings in Bioinformatics, 2001 - academic.oup.com
… need for reliable functional characterisation and annotation of newly predicted proteins. To
… and more effective means of protein sequence characterisation and annotation are required…

Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions

CW Stephen, P Helminen, DP Lane - Journal of molecular biology, 1995 - Elsevier
… Here we describe the characterisation of nine monoclonal antibodies reactive with human
p53 using three phage-display libraries, which express respectively 6-mer, 12-mer and 20-…

A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes

CJ Gloeckner, K Boldt, A Schumacher… - …, 2007 - Wiley Online Library
… Determining functional relationships between proteins in eukaryotic cells is one of the most
… Increasing attention has been directed to the analysis of functional protein complexes. Affinity …

Functional expression and characterisation of membrane transport proteins

I Haferkamp, N Linka - Plant Biology, 2012 - Wiley Online Library
… , has not yet been functionally characterised. Hence, to understand the … transport proteins
have highly improved their functionalprotein expression and functional characterisation using …

The immune diversity in a test tube-non-immunised antibody libraries and functional variability in defined protein scaffolds

E Soderlind, R Carlsson… - … Chemistry & High …, 2001 - ingentaconnect.com
functional library size, as this is considered to be a major factor in the successful use of the
library… intact, correctly folded and functional protein in the expression system being employed. …

A roadmap for gene functional characterisation in wheat

NM Adamski, P Borril, J Brinton, C Marchal… - …, 2019 - repository.rothamsted.ac.uk
functional characterisation of genes in this species. The recent release of a high-quality
reference sequence for wheat underpins a suite of genetic and genomic resources that support …