… their release on fungal pathogenesis, and … fungal EVs remain obscure. In this manuscript,
we will focus our discussion on three fundamental but still unanswered questions about fungal …

… Fungal extracellular vesicles (EVs) were first characterized a decade ago. Knowledge in …
questions remain unanswered, it is now clear that EVs play fundamental biological roles in fungi…

… After our initial description of fungal extracellular vesicles in 2007,Citation12 eight different
studies showing their functions in fungal physiology or pathogenesis have been reported in …

… Although these studies highlight the relevance of EVs in mediating molecule exchange
from plant to fungi, an unanswered question is whether fungi use EVs to deliver effector …

… The biological functions of fungal extracellular vesicles (EVs) or exosomes have … questions
about the cellular origin and mechanisms of biogenesis of fungal EVs remain unanswered. …

… of extracellular vesicles (EVs) in Gram-negative bacteria. However, for cell-walled microbes
such as fungi, … In this review, we address the issues raised by skeptics while simultaneously …

… Recent findings have revealed a role for fungal vesicles in … current understanding of fungal
extracellular vesicles, including … response and some key questions that remain unanswered. …

… However, important questions remain unanswered, as discussed in the previous topics of
this manuscript. Additional studies on the functions, biogenesis and methods of analysis of …

… properties of fungal EVs. Their functionality and the still unanswered questions are presented,
… mechanisms through the fungal cell wall and the importance and packaging of the cargo. …

… focus on vesicular mechanisms of secretion in fungi, highlighting the … of how fungal molecules
reach the extracellular space. … Several questions, however, remain unanswered, and some …