… of genetic networks in mammalian cells is still painstakingly slow and fraught with
inexplicable failures. Here, we demonstrate that transiently expressed genes in mammalian cells …

… With this framework as a tool, we believe that performance issues attributed to gene
expression burden can be addressed head-on in the design phase of circuit-building, thereby …

… how it can lead to gene expression coupling, gene expression and metabolic burden. We
also examine ways to quantify cellular burden in mammalian cells, and investigate circuit-…

… burden resulting from the increased expression of selection genes affects the growth of
mammalian cells… used for the relative quantification of mRNA expression levels. The values of the …

… of genetic circuits in synthetic biology by identifying resource limitations in mammalian cells
… could help advance genetic circuit engineering in mammalian cells and could contribute to …

… In mammalian cells, there tends to be a modest correlation … expression and growth rate
indicating that metabolic burden … HC mRNA expression during production with cells transfected …

… metabolism and placing a metabolic load (metabolic drain, metabolic 'burden) on the host. …
Manufacture of biopharmaceutical proteins by mammalian cell culture systems. Biotechnol. …

… influencing the expression of causal genes might … a burden of rare variants with gene
expression itself, focusing on just the promoter regions of a targeted set of genes whose expression …

… burden caused by lacZ gene expression, which was evaluated separately from the effect
of neo gene expression, must be negligible, as higher expression … type mammalian cell …

… major burden in maintaining homeostasis with respect to distinct stressful conditions. The
prominent role of cell-cell signaling in the gene expression responses of individual cell types to …