… been extended to mammalian cells, being … cells specifically silence gene expression upon
treatment with long dsRNAs (≈500 nt). This response shows hallmarks of conventional RNAi …

… as small interfering RNAs (siRNAs) to elicit gene-specific inhibition in mammalian cells has
made RNAi possible in … Stable suppression of gene expression by RNAi in mammalian cells. …

… To determine whether this DNA vector-based approach can be used to inhibit gene
expression in mammalian cells, we first tested DNA template-derived siRNA on a transfected …

… In addition to the methods described in this chapter, there are also at least two other methods
used to induce RNAi in mammalian cells. One consists of transfection with a mix of multiple …

… a gene for JDP2, and we also examined the effects on siRNA activity of chemical modification
and of mismatches on the sense and antisense RNA strands in cultured mammalian cells. …

… As more knowledge is gained about the mechanism of dsRNA-mediated gene regulation
in mammalian cells, it is anticipated that these will be included in future library designs. …

… Silencing of gene expression by RNA interference (RNAi) has become a powerful tool for …
including si/shRNAs against genes that are not expressed in mammalian cells [eg, luciferase, …

… gene off. We used the switch to regulate the expression of EGFP in mouse and human cells
and found that it offers >99% repression as well as the ability to tune gene expression. To …

… of gene expression as a result of transcriptional gene silencing. Pioneering studies in fission
yeast have shown that endogenously expressed … of transcriptional gene silencing complex (…

… In particular, RNA interference (RNAi) has been the subject … gene regulation and utility as
a tool for artificial gene … -transcriptional gene regulation in mammalian cells in which RNAi is …