… gene expression directly in living cells. Furthermore, we have demonstrated that the stably
integrated gene … To examine the dynamics of gene expression in living cells, we developed …

… of gene expression, and to define their temporal and spatial parameters by using this
methodology to label endogenous genes … how imaging gene expression in single living cells can …

… could be used to control gene expression in vivo, we inserted one copy … expression plasmid,
SVβGal (Promega), generating the construct SVH2βgal. Chinese hamster ovary (CHO) cells …

… , we set out to probe gene expression at the single-molecule level by real-time monitoring
of protein production in live cells. Gene expression is often stochastic (11–14), because most …

… Gene Expression System Although it is now possible to visually observe gene expression in
real time in a living cell, this … (“caged”) molecule that induces gene expression. However, the …

… of the mobility of molecules in cell nucleus is presented. Finally we discuss the influence
of crowding on the bimolecular association rates and gene expression in living cells. …

… the expression level of Circuit 2, we demonstrated computationally and experimentally that
indirect coupling between these seemingly unconnected genetic … that enables cells to reliably …

… First, we describe fluorescence imaging of gene expression with a wide variety of … gene
expression. Besides incorporating the reporter gene into the host DNA, mRNA-based BLI of gene …

… expression in transformed cells are limited because they require such harsh conditions that
viability of the cells … for detection of lacZ expression in living cells under standard culture or …

… of temporal gene expression patterns is … live cell imaging of fluorescent protein transcriptional
reporters to achieve real-time characterization of gene expression programs in living cells. …