Dicistronic transcription units for gene expression in mammalian cells

W Dirks, M Wirth, H Hauser - Gene, 1993 - Elsevier
… translation of the two genes. Using internal … expression of both reporter genes and expression
levels comparable to those achieved by the respective monocistronic expression vectors. …

Global quantification of mammalian gene expression control

B Schwanhäusser, D Busse, N Li, G Dittmar… - Nature, 2011 - nature.com
Gene expression is a multistep process that involves the transcription, translation and … pulse
labelling for more than 5,000 genes in mammalian cells. Whereas mRNA and protein levels …

Integrated genomic and proteomic analyses of gene expression in mammalian cells

Q Tian, SB Stepaniants, M Mao, L Weng… - Molecular & Cellular …, 2004 - ASBMB
… data, we compared protein and mRNA expression patterns within clusters 1 or 2. For each
drug and dose combination, we averaged gene expression ratios of all the proteins or mRNAs …

Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector

JP Condreay, SM Witherspoon… - Proceedings of the …, 1999 - National Acad Sciences
gene expression in eukaryotic cells (12); thus, we investigated its effect on expression from
the mammalian … Sodium butyrate was added to CHO cell culture medium at a concentration of …

Ultrasound imaging of gene expression in mammalian cells

A Farhadi, GH Ho, DP Sawyer, RW Bourdeau… - Science, 2019 - science.org
… Here, we describe the expression of ARGs in mammalian cells to enable ultrasound imaging
of mammalian gene expression. To identify a set of genes capable of encoding gas vesicle …

Hypoxia‐mediated regulation of gene expression in mammalian cells

SC Shih, KP Claffey - International journal of experimental …, 1998 - Wiley Online Library
gene expression. In the first part of this review, we will introduce the metabolic state of cells
… in the regulation of hypoxia-responsive gene expression, and the third part will cover the …

Regulated translation initiation controls stress-induced gene expression in mammalian cells

HP Harding, I Novoa, Y Zhang, H Zeng, R Wek… - Molecular cell, 2000 - cell.com
… activates genes involved in amino acid biosynthesis. Mammalian cells thus utilize an
ancient pathway to regulate gene expression in response to diverse stress signals. …

Nutritional control of gene expression: how mammalian cells respond to amino acid limitation

MS Kilberg, YX Pan, H Chen… - Annu. Rev. Nutr., 2005 - annualreviews.org
▪ Abstract The amino acid response (AAR) pathway in mammalian cells is designed to
detect and respond to amino acid deficiency. Limiting any essential amino acid initiates this …

Differential effects of polyadenylation regions on gene expression in mammalian cells

DS PFARR, LA RIESER, RP WOYCHIK, FM ROTTMAN… - Dna, 1986 - liebertpub.com
mammalian cells. Our results demonstrate that the presence of a poly(A) region is important
for efficient gene expression … results in three times higher expression than that of SV40 early …

Vectors for gene expression in mammalian cells

SC Makrides - New comprehensive biochemistry, 2003 - Elsevier
… the host cell, and the genetic properties of the host. Gene transfer into mammalian cells may
be effected either by infection with virus that carries the recombinant gene of interest, or by …