… To determine whether this DNA vector-based approach can be used to inhibit gene
expression in mammalian cells, we first tested DNA template-derived siRNA on a transfected …

… expression vectors, both non-viral and viral, have been designed to transfer foreign genes
into mammalian cells. The choice of the vector depends on the application, the host cell, the …

… of gene products at steady state. This kinetic model suggests that sensitivity of gene products
to … and proteinases is an important determinant of the time scale of gene expression. …

… cellular processes. Then, we focus on recent developments in light-controlled gene
expression … the reader with a guideline for choosing the most suitable gene expression system. …

… gene regulation technology in mammalian cells is whether to use an inducible “ON”-type (target
gene expression … ”-type system (target gene expression is repressed upon addition of …

… the positive gene delivery properties of PEI observed in adherent cells … transient gene
expression system with mammalian cells. … gene expression in mammalian cells in serum-free sus- …

… , to follow the dynamics of gene expression in transiently transfected mammalian cells.
Gene transfer conditions for lipofection were optimized. The highest fraction of transfectants …

… and show that a threshold expression level is required to induce apoptosis. This work … of
mammalian gene expression that can be used to explore the functional role of various genes as …

The transfer of foreign genes into eukaryotic cells, in particular mammalian cells, has been …
significance of genes and regulatory sequences as well as the development of gene therapy …

… The technology of expressing foreign genes in mammalian cells has … Mammalian cells are
frequently used as a host for … Mammalian cells are used as a host for gene expression in order …