Systematic evaluation of site-specific recombinant gene expression for programmable mammalian cell engineering

N Pristovsek, S Nallapareddy, LM Grav… - ACS synthetic …, 2019 - ACS Publications
… To demonstrate the usefulness of our toolbox for recombinant protein production in
mammalian cell lines, we aimed to characterize the expression levels of a secreted …

How to choose the right inducible gene expression system for mammalian studies?

T Kallunki, M Barisic, M Jäättelä, B Liu - Cells, 2019 - mdpi.com
… down and knock out gene expression, respectively, in mammalian cells. RNA interference
(RNAi) has emerged as an essential tool to achieve knockdown of gene expression [31,32]. It …

[PDF][PDF] Coordination of gene expression in the formation of mammalian mitochondria

P Nagley - Trends in Genetics, 1991 - academia.edu
… feature of the mammalian cellular landscape, these genes have a general … cells. In
circumstances where the mitochondrial contribution to cellular activities is modulated, such as …

Differential regulation of polo-like kinase 1, 2, 3, and 4 gene expression in mammalian cells and tissues

JA Winkles, GF Alberts - Oncogene, 2005 - nature.com
… , 2, 3, or 4 gene expression either during mammalian cell cycle progression or in tissue
samples. These studies have demonstrated that the Plk family genes are differentially expressed

Light control of the Tet gene expression system in mammalian cells

M Yamada, Y Suzuki, SC Nagasaki, H Okuno… - Cell reports, 2018 - cell.com
gene expression system in mammalian cells. Most light-inducible gene expression systems
were established in yeast cells. … -inducible gene expression systems optimized in yeast cells

Negative and Positive Regulation in trans of Gene Expression from Adeno-Associated Virus Vectors in Mammalian Cells by a Viral rep Gene Product

JD Tratschin, J Tal, BJ Carter - Molecular and Cellular Biology, 1986 - Taylor & Francis
expression in mammalian cells of the gene for chloramphenicol acetyltransferase (CAT). In
the AAV vector, pTS1, the CAT gene is expressed … to regulate gene expression. First, deletion …

Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors

I Shoji, H Aizaki, H Tani, K Ishii… - Journal of General …, 1997 - microbiologyresearch.org
… not replicate in mammalian cells. We then compared the efficiencies of gene expression by
the … of gene expression by baculovirus vectors in mammalian cells, we compared luciferase …

Stochastic mRNA synthesis in mammalian cells

A Raj, CS Peskin, D Tranchina, DY Vargas… - PLoS biology, 2006 - journals.plos.org
gene expression. In this study, we explored cell-to-cell variation in gene expression in
mammalian cells by … ) products of gene expression, we show direct evidence that genes transition …

Mammalian cell protein expression for biopharmaceutical production

J Zhu - Biotechnology advances, 2012 - Elsevier
… new cell lines, introducing novel genetic mechanisms in expression, gene silencing, and
gene … Additionally, transient gene expression technology platform plays more important role in …

Gene silencing in mammalian cells and the spread of DNA methylation

MS Turker - Oncogene, 2002 - nature.com
gene expression. The last section of this review will deal with what happens when silencing
begins, and the blocking force is weakened thereby allowing the spread of methylation …