Normalization

J Huggett, K Dheda, SA Bustin - Real-time PCR, 2007 - taylorfrancis.com
… Both of these pose serious problems for gene expressionnormalization, address the
associated problems when using real-time PCR and discuss data interpretation once normalization

A new normalization for Nanostring nCounter gene expression data

R Molania, JA Gagnon-Bartsch, A Dobrovic… - Nucleic acids …, 2019 - academic.oup.com
normalization methods, but that we should focus more on assessing the effectiveness of any
normalization … we present our novel normalization for nCounter gene expression data, but in …

[HTML][HTML] Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.)

R Huis, S Hawkins, G Neutelings - BMC plant biology, 2010 - Springer
… and their expressiongene are necessary for normalizing gene expression when all studied
samples are considered. However, only 2 TEFs are required for normalizing expression in …

Normalization methods for analysis of microarray gene-expression data

YJ Chen, R Kodell, F Sistare… - Journal of …, 2003 - Taylor & Francis
… Since the list of genes that are differentially expressed is not known, the commonly applied
global normalization method uses all genes on the array for adjustment. Global normalization

[HTML][HTML] Evaluation of Candidate Reference Genes for Gene Expression Normalization in Brassica juncea Using Real Time Quantitative RT-PCR

R Chandna, R Augustine, NC Bisht - PloS one, 2012 - journals.plos.org
… To validate the selection of candidate reference genes for normalization, we analyzed the
normalized fold expression of BjDREB2 (a dehydration responsive element binding proteins-2 …

[HTML][HTML] Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review

J Lü, C Yang, Y Zhang, H Pan - Frontiers in physiology, 2018 - frontiersin.org
… measurements of gene expression levels. Here, … gene selection for insect gene expression
studies that employed the most widely used SYBR Green method for RT-qPCR normalization. …

[HTML][HTML] Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR

H Ling, Q Wu, J Guo, L Xu, Y Que - PloS one, 2014 - journals.plos.org
… In this study, eEF-1a, which has been proven to be a suitable reference gene for expression
normalization in O. sativa and C. sativus [5], [12], also ranked at the top when evaluated by …

[HTML][HTML] Identification of suitable reference genes for gene expression normalization in qRT-PCR analysis in watermelon

Q Kong, J Yuan, L Gao, S Zhao, W Jiang, Y Huang… - PloS one, 2014 - journals.plos.org
… In addition, the identified reference genes were expressed at much lower levels … normalization
of gene expression over a wide range of transcript levels. The identified reference genes

Impact of normalization on miRNA microarray expression profiling

S Pradervand, J Weber, J Thomas, M Bueno… - Rna, 2009 - rnajournal.cshlp.org
normalization methods for mRNA gene expression arrays are well established, miRNA array
normalization … In this study we investigate the impact of normalization on data generated …

[HTML][HTML] Evaluating different methods of microarray data normalization

A Fujita, JR Sato, LO Rodrigues, CE Ferreira… - BMC …, 2006 - Springer
… The SVR method is shown to be very robust to outliers presented at different gene
expression levels, becoming the best normalization technique to identify actual differentially …