… the importance of the real-time PCR (qRT-PCR) as a technique for mRNA expression analysis
in different tissues. Real-time PCR is widely used for quantification of mRNA levels and is …

… Principles of quantitative real time PCR using fluorogenic probes: scheme … normalization
of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes…

… of the performance of ten conventional housekeeping genes across 21 soybean samples
allowed the identification of a panel of genes suitable for gene expression normalization [36]. …

… In contrast, quantitative reverse transcriptase real-time PCR (QRT-PCR) is more robust and
… expression measurements by reverse transcription of RNA followed by quantitative real-time …

… normalized gene expressions as either means of Normalized Expressions or Mean Normalized
Expressions. In … that the calculation of Mean Normalized Expressions has to be used for …

… , namely quantitative PCR (qPCR-quantitative real-time … gene expression, due their ability
to rapidly and accurately quantify target genes, even in the presence of very low expression …

… quantitative real-time PCR (qPCR) based techniques have become essential for gene
expression … Normalizing to reference gene in relative quantification make results from qPCR more …

… , the normalization of quantitative real-time PCR is important to obtain accurate gene expression
… Quantitative real-time PCR (qPCR) is a technique that is frequently used to measure the …

… In order to select the most suitable RG for gene expression quantification by RT-qPCR, we
analysed several tissues of cork oak including leaves, reproduction cork and periderm from …

… for quantification of genes with low expression levels. Therefore, we recommend the use
of UBQ5 and/or eEF-1α as suitable reference gene for normalization of gene expression in a …