… Here, we show that lin-28 is regulated posttranscriptionally by two genetic circuits, one
involving the 22-nt RNA lin-4 and one that is independent of lin-4. Both of these circuits appear to …

… and strength are usually more readily found in nucleic-acid-based devices than they are
in protein-based molecular devices, making RNA attractive for gene circuit design 28,29 . …

… Curation of genetic circuit components and the development of … of genetic circuits in
eukaryotic cells, we present BLADE, a general framework for engineering complex logic circuits in …

… The behavior of genetic circuits, and their response to pharmacological perturbations, …
cells and help to inform our understanding of the basic design principles of genetic circuits. …

… Here, we present a proof-of-concept immunomodulatory gene circuit platform that enables
tumor-specific expression of immunostimulators, which could potentially overcome these …

… homologue of lin-4; ref. 8) has been shown to regulate the mouse lin-28 homologue 9 , a
marker for pluripotency in human ES cells. lin-28 is one of four genes that cooperate to induce …

… in the lin-28 gene contribute to its downregulation in differentiating P19 cells, we compared
the expression of a luciferase reporter gene bearing the entire lin-28 3′ UTR (Luc-lin 28) to …

… Our approach enables the construction of tunable gene circuits in complex eukaryotic … We
focused on three genetic circuits for detailed analysis. For each genetic circuit, we chose one …

… cooperative assemblies can program nonlinear gene circuit behavior in yeast. Using a model…
-input circuits. We demonstrate that assemblies can be adjusted to control circuit dynamics. …

… tools available for engineering gene circuits in microbes, including … scalable circuit performance
such as insulating a gene circuit … causes of failure and fine-tuning circuit characteristics. …