… model of eukaryotic gene expression which accounts for resource loading, including
squelching by TAs. To solve the resource loading problem in mammalian cells, we introduce a …

… We assayed the behavior in mammalian cells of large gene circuits constructed with our
method. A 39-kb vector containing a 7-TU circuit that was assembled into a BAC carrier vector ( …

… of synthetic gene networks, types of building modules, … when guided to the gene promoter
region by sterically hindering … way to silence gene expression in mammalian cells is through …

… mammalian cellular engineering. A major asset of the MTK is a large, characterized suite of
modular parts to build versatile TUs, which we showcased by driving different levels of gene …

… of sophisticated functions in mammalian cells. A particularly … strategies available for genetic
programming in mammalian cells. We … modules that each perform one specific function, and …

… not currently available for mammalian cells. It is … of genetic circuits in eukaryotic cells, we
present BLADE, a general framework for engineering complex logic circuits in mammalian cells …

… relationship between the PAF complex and PARP modules, we treated cells that are
depleted for PAF complex function (using RNAi-mediated knockdown of Rtf1) with the PARP …

… in two types of cocultured cancer cells with greatly improved … genetic parts for modular
assembly of synthetic circuits with predictable and programmable behavior in mammalian cells for …

… for DNA construction for mammalian cells. We compare the … now dominate due to their
modular design. We look forward to … modular construction of biological circuits in mammalian cells…

… mammalian cell-compatible expression control systems laid the foundations for the assembly
of transcription control modules … family for tricistronic gene expression in mammalian cells. …