… Transposase enzymes can integrate recombinant genes into the genomes of mammalian
cell lines. When transposases recognize short inverted DNA sequences, they excise DNA that …

… Other applications include engineering of cell lines for drug screening and cell-based …
genetic technologies and emerging strategies for genetically engineering mammalian cells …

… optimize transfection conditions for specific cellular strains. In this study, we utilized plasmids
with green fluorescent protein (GFP) encoding genes as a model in order to optimize … of cell …

… for preparing codon-optimized genes for expression it in mammalian cells by selection of …
human codon-optimized method”. Based on our simple design rule for codon optimization, we …

… originating from the complexity of the mammalian cell culture processes. An alternative is …
genetic algorithm as a method of experimental design for optimization of mammalian cell …

… Advances in media- and process optimization have already resulted in more than … genetically
modify mammalian cells mainly rely on the overexpression of exogenous effector genes …

… Mammalian cells are essential to perform such modifications correctly, and efficient … be
optimized to increase gene expression. An approach based on transfection of mammalian cells …

… Therefore, genetically engineered CYP expression systems … In this context there is a clear
need for genetically engineered … conditions of the human hepatocyte systems are optimized. …

… optimize the multicomponent expression system for UAA incorporation into proteins in
mammalian cells… level of the encoded genes in a population-wide manner. Furthermore, the entire …

… of recombinant proteins in mammalian cell lines has become … by expression in a mammalian
cell is relevant to all classes … transcriptional activity of integrated genes with a concomitant …