… construct using banana codon-optimized Cas9 and pMaU6c (700 bp). To determine the activity
of the new CRISPR/Cas9 system for banana endogenous gene editing, … of the optimized …

… We also discuss strategies for the design of multiplex CRISPR constructs for … constructs
and discuss the remaining challenges of CRISPR construct optimization in plant genome editing…

… HDR gene-editing events, which we refer to as Genome Editing Assessment System (GEAS).
First, we designed and constructed … We then transfected the nuclease constructs and donor …

… multiplexed NGS, it is possible to perform fast functional screens for a large number of gRNAs
and to optimize constructs for effective editing of multiple independent targets in the wheat …

… To compare the levels of sgRNA expression achieved by the proGhU6.3.3 and proAtU6-29
in cotton, we constructed two CRISPR vectors using each promoter to drive a previously …

… precise genomic editing for human biomedical studies, we tested functionality of our targeted
deaminases in human cells. We constructed a … We designed and optimized the structure of …

… was constructed by … the genome editing efficiency with our optimized gRNA in the sites that
the conventional gRNA shows low genome editing efficiency; second, improving the genome …

… 4), we constructed heat maps presenting the on- and off-target activities conferred by the …
The activity-optimized variant Opti-SpCas9 differs from WT SpCas9 by two substitutions at DNA-…

… Optimized ssODNs are constructed with an asymmetric distribution of homology and a
polarity determined by the position of the DSB relative to the intended integration site. …

… AcPDS gene, respectively, and both were further constructed … sites to form a new Cas9 binary
construct, which we designated as … the two AscI sites to construct another new Cas9 binary …