Genome sequencing of sewage detects regionally prevalent SARS-CoV-2 variants

A Crits-Christoph, RS Kantor, MR Olm, ON Whitney… - MBio, 2021 - Am Soc Microbiol
genetic diversity across infected communities. Here, we sequenced RNA directly from sewage
… to generate complete and nearly complete SARS-CoV-2 genomes. The major consensus …

The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019

G Fongaro, PH Stoco, DSM Souza, EC Grisard… - Science of The Total …, 2021 - Elsevier
… amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was
… based on PEG used for SARS-CoV-2 concentration in human raw sewage in this study, had …

Genome sequencing of SARS-CoV-2 allows monitoring of variants of concern through wastewater

M Herold, AF d'Hérouël, P May, F Delogu… - Water, 2021 - mdpi.com
… from individual SARS-CoV-2 lineages in wastewater samples to … the potential of genomic
surveillance in wastewater samples … basis for the early detection of novel SARS-CoV-2 variants. …

Rapid genomic surveillance of SARS-CoV-2 in a dense urban community using environmental (sewage) samples

R Napit, P Manandhar, A Chaudhary, B Shrestha… - MedRxiv, 2021 - medrxiv.org
… The obtained consensus genome sequences of SARS-CoV-2 from the sewage samples
identified B.1, B.1.36 and B1.1 as circulating lineage. The lineage B.1 was a strain circulating in …

Tracking SARS-CoV-2 in sewage: evidence of changes in virus variant predominance during COVID-19 pandemic

J Martin, D Klapsa, T Wilton, M Zambon, E Bentley… - Viruses, 2020 - mdpi.com
… Whole-genome sequences were aligned to a reference sequence (Wuhan-Hu-1 strain)
with National Center for Biotechnology Information (NCBI) accession no. MN908947 and the …

[HTML][HTML] Use of sewage surveillance for COVID-19 to guide public health response: A case study in Hong Kong

Y Deng, X Xu, X Zheng, J Ding, S Li, HK Chui… - Science of the Total …, 2022 - Elsevier
… ) sequencing platforms, and compared the obtained sequences to the whole genome sequences
… By comparing to the reference genome sequence of a SARS-CoV-2 virus isolate strain (…

[HTML][HTML] Monitoring SARS-CoV-2 circulation and diversity through community wastewater sequencing, the Netherlands and Belgium

R Izquierdo-Lara, G Elsinga, L Heijnen… - Emerging infectious …, 2021 - ncbi.nlm.nih.gov
… Whole-genome sequencing of SARS-CoV-2 from clinical samples has been adopted as
an additional tool to identify clusters. Particularly in geographic areas with minimal …

Rapid increase of SARS-CoV-2 variant B. 1.1. 7 detected in sewage samples from England between October 2020 and January 2021

T Wilton, E Bujaki, D Klapsa, M Majumdar, M Zambon… - Msystems, 2021 - Am Soc Microbiol
… Selected PCR products from nPCR reactions A and B were analyzed by Sanger sequencing
to confirm the SARS-CoV-2 genome sequence. nPCR product B contains codon GAT at …

[HTML][HTML] Exploring the diversity of coronavirus in sewage during COVID-19 pandemic: Don't miss the forest for the trees

S Martínez-Puchol, M Itarte, M Rusiñol, E Forés… - Science of the Total …, 2021 - Elsevier
… correlation between the concentration of SARS-CoV-2 in wastewater and the prevalence
of … % for clinical sample B, of the 29,903 bp of reference SARS-CoV-2 genome sequence. …

Metagenomic sequencing of municipal wastewater provides a near-complete SARS-CoV-2 genome sequence identified as the B. 1.1. 7 variant of concern from a …

C Landgraff, LYR Wang, C Buchanan, M Wells… - MedRxiv, 2021 - medrxiv.org
SARS-CoV-2 sequence from a wastewater … to the SARS-CoV-2 B.1.1.7 variant of concern.
Our results demonstrate that a combined interrogation of genome consensus-level sequences