Analysis of the microbiome: Advantages of whole genome shotgun versus 16S amplicon sequencing

R Ranjan, A Rani, A Metwally, HS McGee… - … and biophysical research …, 2016 - Elsevier
… (rRNA) gene. We present a comparative study of an alternative approach using … genome
shotgun sequencing (WGS). In the present study, we analyzed the human fecal microbiome

[HTML][HTML] Colorectal cancer and the human gut microbiome: reproducibility with whole-genome shotgun sequencing

E Vogtmann, X Hua, G Zeller, S Sunagawa, AY Voigt… - PloS one, 2016 - journals.plos.org
… In this study, we were able to use whole-genome shotgun metagenomic sequencing to …
16S rRNA gene sequencing [2]. The current study also reproduced some significant gene-level …

[HTML][HTML] Use of whole genome shotgun metagenomics: a practical guide for the microbiome-minded physician scientist

J Ma, A Prince, KM Aagaard - Seminars in reproductive …, 2014 - thieme-connect.com
… [5] [6] [7] [8] Further, dysbiosis of the microbiome has been implicated as a cause for preterm
… a healthy microbiome. With the exponentially expanding interest in human microbiome

Whole-genome shotgun metagenomic sequencing reveals distinct gut microbiome signatures of obese cats

X Ma, E Brinker, EC Graff, W Cao, AL Gross… - Microbiology …, 2022 - Am Soc Microbiol
… the whole-genome metagenomic level. We performed whole-genome shotgun metagenomic
… In the obese cat microbiome, we discovered a significant reduction in microbial diversity (P …

[HTML][HTML] Reconstructing the genomic content of microbiome taxa through shotgun metagenomic deconvolution

R Carr, SS Shen-Orr, E Borenstein - PLoS computational biology, 2013 - journals.plos.org
… is a technique for associating genomic elements found in shotgun metagenomic samples
with their taxa of origin and for reconstructing the genomic content of the various taxa …

Comparison of methods to collect fecal samples for microbiome studies using whole-genome shotgun metagenomic sequencing

DA Byrd, R Sinha, KL Hoffman, J Chen, X Hua, J Shi… - Msphere, 2020 - Am Soc Microbiol
… hg38 human reference genome, and all remaining reads were mapped to a custom database
that included all bacterial and archaeal whole-genome shotgun assemblies available at …

[HTML][HTML] Characterization of the gut microbiome using 16S or shotgun metagenomics

J Jovel, J Patterson, W Wang, N Hotte… - Frontiers in …, 2016 - frontiersin.org
… the analyses of bacterial NGS datasets. This article provides a compendium of good practices
for the analysis of NGS microbiome … Using gut microbiome datasets specially designed to …

[HTML][HTML] Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy

S Campanaro, L Treu, PG Kougias… - Biotechnology for …, 2016 - Springer
… genes and extracting 106 microbial genomes by a novel strategy … The organization of the
anaerobic digestion microbiome is … Key microbial genomes encoding enzymes involved in …

Tools for analysis of the microbiome

J Galloway-Peña, B Hanson - Digestive diseases and sciences, 2020 - Springer
… portion of microbial genomes. Shotgun metagenomics approaches consist of methods that
utilize untargeted sequencing methods to capture all microbial genomes present within a …

A clinician's guide to microbiome analysis

MJ Claesson, AG Clooney, PW O'toole - … Reviews Gastroenterology & …, 2017 - nature.com
gene analysis or metagenomic sequencing. Marker gene analysis differs from metagenomic
shotgun sequencing as it is based on targeting an amplicon of only one gene instead of …