High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system
… hirsutum-Base Editor 3 (GhBE3) base-editing system has been developed to create
single-base … performed effective base editing in the target editing window of sgRNA sites. Since …
single-base … performed effective base editing in the target editing window of sgRNA sites. Since …
Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
… Therefore, high-throughput deep sequencing (~ 10 million reads per locus) was also applied
to analyze the A to G base editing profiles for all the edited plants in this report. Through …
to analyze the A to G base editing profiles for all the edited plants in this report. Through …
Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture
G Wang, F Wang, Z Xu, Y Wang, C Zhang, Y Zhou… - Genome Biology, 2024 - Springer
… The Gossypium hirsutum genotype Jin668 was used in this study [62]. All constructs were
introduced into Agrobacterium strain GV3101 (kanamycin as a selectable marker) via …
introduced into Agrobacterium strain GV3101 (kanamycin as a selectable marker) via …
[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)
B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
… As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n = …
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n = …
High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system
P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
… Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …
CRISPR/Cas9: A New Genome Editing Tool to Accelerate Cotton (Gossypium spp.) Breeding
… Inadequate achievement in pioneering desired genes from diploid species to Gossypium
hirsutum is because of obscurity in the F1 hybrid production, chromosome number resurgence …
hirsutum is because of obscurity in the F1 hybrid production, chromosome number resurgence …
Precise plant genome editing using base editors and prime editors
… This Review focuses on base editing and prime editing platforms … base editing platforms
in both nuclear and organelle genomes. We then navigate to recently developed prime editing …
in both nuclear and organelle genomes. We then navigate to recently developed prime editing …
The CRISPR/Cas9 revolution continues: from base editing to prime editing in plant science
Y Li, W Li, J Li - Journal of Genetics and Genomics, 2021 - Elsevier
… latest breakthroughs in precise genome editing (eg, base editing and prime editing), and we
… editing efficiency, expand the targeting scope, and improve the specificity of base editing in …
… editing efficiency, expand the targeting scope, and improve the specificity of base editing in …
[HTML][HTML] Base editing in plants: Current status and challenges
SS Bharat, S Li, J Li, L Yan, L Xia - The Crop Journal, 2020 - Elsevier
… in many laboratories, base editing is becoming an efficient … CBE and ABE base editors in
precise base editing, the two … mechanisms of base editors and their applications in plants. …
precise base editing, the two … mechanisms of base editors and their applications in plants. …
[HTML][HTML] Base editing in plants: applications, challenges, and future prospects
MK Azameti, WP Dauda - Frontiers in plant science, 2021 - frontiersin.org
… CBE was also recently employed in Cotton (Gossypium hirsutum). In a study conducted by
Qin et al. (2020), … hirsutum-Base Editor 3 (GhBE3) base-editing approach, which was used to …
Qin et al. (2020), … hirsutum-Base Editor 3 (GhBE3) base-editing approach, which was used to …
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