… tested as internal control for reliable qPCR data normalization. In addition, to illustrate the
use of cotton reference genes we checked the expression of two cotton MADS-box genes in …

… Real-time PCR methods have been developed to monitor commercial transgenic cotton in
… detection system for cotton, we developed a TaqMan real-time PCR method to determine …

… In the present study reverse transcriptase real time PCR technique was used to monitor the
relative transcript abundance of some cotton genes using SYBR green fluorescent dye. For …

… 35S promoter and introduced into Gossypium hirsutum. The integration and expression …
; PCR from genomic DNA, and quantitative real-time PCR analysis. Transgenic cotton plants …

… Although some miRNAs have been reported as reference genes in different cotton tissues […
through RT-qPCR method. On the basis of our previous RNA-Seq data of Gossypium hirsutum…

… were correct, we designed gene-specific primers for RT-PCR from cotton leaves. The products
range in size from 0.9 to … Overview of six NAC genes identified in Gossypium hirsutum L. …

… In the present study, semi-quantitative RT–PCR was carried out using total RNA extracted
from various tissues at the seedling stage to investigate the expression pattern of GhRDR6 in …

… lack of transcripts GhExp2 at 24 DPA is likely to be a result of failure of the reamplification
step in the RTPCR, as both 22- and 26-DPA templates were successfully amplified. …

… cotton were monitored using microarray and real-time PCR (… ® Cotton Genome Array
representing 21,854 cotton transcripts … validated our results with real-time RT-PCR. The biological …

… as microarray and Northern blot, quantitative real time PCR (qRT-PCR) is a more powerful
technique … Therefore, qRT-PCR has become the best method to validate whether the selected …