Sperm DNA fragmentation in the total and vital fractions before and after density gradient centrifugation: Significance in male fertility diagnosis

U Punjabi, H Van Mulders, I Goovaerts, K Peeters… - Clinical …, 2018 - Elsevier
… inversely correlated with sperm DNA fragmentation in the total … Sperm DNA fragmentation
was observed both in normal and … density gradient centrifugation, sperm DNA fragmentation

Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa

M Muratori, N Tarozzi, F Carpentiero, S Danti… - Scientific reports, 2019 - nature.com
… Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation
… are identified with higher accuracy detecting DNA fragmentation in viable (viable sDF) …

[HTML][HTML] Comparison of the DNA fragmentation and the sperm parameters after processing by the density gradient and the swim up methods

I Amiri, M Ghorbani, S Heshmati - Journal of clinical and diagnostic …, 2012 - ncbi.nlm.nih.gov
… The aim of the present study was to compare the effects of the density-gradient
centrifugation and the swim-up methods on the sperm parameters and the DNA fragmentation. …

… with the simultaneous assessment of cell viability in a subfertile population: Establishing thresholds of normality both before and after density gradient centrifugation

U Punjabi, H Van Mulders, I Goovaerts… - Journal of assisted …, 2019 - Springer
… The aims of this study were to assess the incidence of total and vital sperm DNA fragmentation
… a standardized TUNEL methodology both before and after density gradient centrifugation. …

… of viable human spermatozoa with low levels of DNA fragmentation from an immotile population using density gradient centrifugation and magnetic‐activated cell …

H Zhang, X Xuan, S Yang, X Li, C Xu, X Gao - Andrologia, 2018 - Wiley Online Library
… was used to determine the extent of DNA fragmentation in our patient samples and was
carried out with a Fluorescein FragEL™ DNA Fragmentation Detection kit. Sperm samples were …

Semen processing by density gradient centrifugation is useful in selecting sperm with higher double‐strand DNA integrity

S Brahem, M Mehdi, H Elghezal, A Saad - Andrologia, 2011 - Wiley Online Library
… In conclusion, our data support a significant correlation between sperm DNA fragmentation
and strict spermatic morphology. In addition, we have shown that semen processing by the …

Efficacy of swim-up versus density gradient centrifugation in improving sperm deformity rate and DNA fragmentation index in semen samples from teratozoospermic …

X Xue, WS Wang, JZ Shi, SL Zhang, WQ Zhao… - Journal of assisted …, 2014 - Springer
… DGC in improving sperm deformity and DNA fragmentation and to determine which method
is … morphology and DNA fragmentation, and analyzed the correlation between DNA integrity …

The effects of different sperm preparation methods and incubation time on the sperm DNA fragmentation

XD Zhang, MY Chen, Y Gao, W Han, DY Liu… - Human …, 2011 - Taylor & Francis
… on sperm DNA fragmentation. Methods: Sperm DNA fragmentation was assessed by sperm
chromatin dispersion (SCD) method on motile sperm prepared by gradient centrifugation or …

[HTML][HTML] Effects of semen storage and separation techniques on sperm DNA fragmentation

RE Jackson, CL Bormann, PA Hassun, AM Rocha… - Fertility and sterility, 2010 - Elsevier
… The significant reduction in fragmentation noted in our density gradient centrifugation and …
density gradient centrifugation and swim-up techniques can reduce DNA fragmentation rates, …

Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up …

YN Yang, L Wang, YB Liu, YJ Xue, CC Liu… - Reproductive and …, 2022 - journals.lww.com
… been introduced as an alternative to conventional centrifugation-based techniques [ 14–16
] … levels of DNA fragmentation [ 17 ] . Nevertheless, density gradient centrifugation (DGC) in …