grxB gene as a potential target for the development of Cronobacter sakazakii detection method in infant formula milk using real-time PCR
Cronobacter sakazakii is a pathogen that causes necrotizing enterocolitis, sepsis, and
meningitis in infants or neonates, with reported case-fatality rates varying from 40 to 80
percent. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii.
This study aims to get the primer pairs and optimal annealing temperature for the
Polymerase Chain Reaction (PCR) method to detect C. sakazakii. The glutaredoxin B (grxB)
gene was found suitable for detecting Cronobacter sakazakii, and a primer pair based on …
meningitis in infants or neonates, with reported case-fatality rates varying from 40 to 80
percent. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii.
This study aims to get the primer pairs and optimal annealing temperature for the
Polymerase Chain Reaction (PCR) method to detect C. sakazakii. The glutaredoxin B (grxB)
gene was found suitable for detecting Cronobacter sakazakii, and a primer pair based on …
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