… HA refers to the high affinity binding site, and LA refers to the low affinity … the high affinity
Ca 2+ binding site (K m = 1.2 μM), we calculate ΔG W,HA = −14.9 kT, and for the low affinity site (…

… cation and ouabain binding sites of the high-affinity ouabain complex with Mg 2+ -bound at
site II (Left) and the K + -occluded low-affinity … of the CTS binding site as a consequence of the …

… of several of these cation transport enzymes, however, no high affinity cation binding
sites have yet been determined. Here we report the use of oligonucleotide-directed, site-specific …

… In our attempts to elucidate whether high-affinity cation binding sites may participate in
cation transport, we also measured the concentration dependence of [ 3 H]MPP uptake at low …

… a single high affinity, cation-selective binding site, flanked by low affinity, nonselective sites.
This … a single high affinity binding site, where selectivity and ionic modulation of gating occur. …

… it is generally thought that a cation site near the retinal Schiff base is … cation binding on the
visible absorption maximum; 2) the change in number and affinities of the cation-binding sites …

… present suggests that divalent cation binding sites on platelets are heterogeneous (20, 27). …
with the presence of divalent cation binding sites on platelets distinct from the high affinity …

… The protein actin has a single high affinity binding site for a divalent cation, usually … cation
exchange at the high affinity site is markedly affected by concurrent low affinity cation binding. …

… sites on actin at the same … high-affinity nucleotide binding site on G-actin (16). The separation
of ribose and adenine protons on the bound ATP from the high-affinity cation binding site …

… ( 8 ) ,Ca2+ and Mg2+ previously reported (Frieden, C., Lieberman, D., and compete for a
single high-affinity binding site for which we Gilbert, HR (1980) Biol. Chem. 255,8991-8993). …