… use of sequencing as a practical tool for mutation analysis. While in the past the high cost of
sequencing limited mutation analysis to selectable markers or small forward mutation targets …

… to directly analyze the genetic material in primary cells or tissues of any species in a high
throughput manner … cell lane, it is possible to perform genome-wide, point mutation analysis by …

… The article is designed to provide an analytic roadmap for variant analysis, thereby enabling
a wide range of researchers and clinical genetics practitioners to perform direct analysis of …

… In our analyses, genomic DNA samples were directly extracted from heterogeneous …
mutational analyses with a reduced amount of sequence data using sub-sampled sequence reads …

… ’ for mutation analysis is DNA sequencing. However, for … for mutation detection in DNA are
based on analysis of either the DNA sequence directly (eg, by methods based on sequencing …

… (C) Samples are submitted for high-throughput sequencing on a HiSeq2000 Illumina
platform performing a paired-end run of 2×100 bases. Fluidigm custom primers are used for …

… Therefore, we tested a high-throughput screening method for acute myeloid leukemia
mutation analysis using a … All suspected mutations were confirmed by direct DNA sequencing. …

… previously analyzed by direct sequencing for mitochondrial DNA … mutation analysis—75
tumors encompassing >2 million bases of mitochondrial DNA were sequenced and analyzed by …

The gDNA yield of 2.7–28 µg from the saliva samples was sufficient for mutation analysis.
The variation in the amount mostly depended on the original volume of saliva. Gel …

… frequency and spectrum of sequence alterations outside these … high-throughput DNA
sequencing of all coding exons of FLT3 in AML patients without FLT3 ITDs or known AL mutations. …