… respiratory epithelial cells, without preculture manipulations, … to generate airway epithelial
organoids (tracheobronchospheres) … strategy to generate 3-D airway cultures. Furthermore, the …

… Here, we describe a versatile approach to establishing adult human airway epithelial
organoids, containing all major cellular elements. Major differences to other lung organoid models …

… of PSC-derived airway organoids. To generate functional airway organoids, PSCs are …
definitive endoderm and lung progenitor stages) into mature airway epithelium [25-36]. Over the …

… Airway organoids can be grown from stem/progenitor cells of different parts of the lung, and
… for establishing human airway organoids have been described (3, 4). Airway organoids are …

… Collectively, we developed differentiated human airway organoids which can morphologically
and functionally simulate human airway epithelium. These differentiated airway organoids …

… , which in many cases exceeds the iPSCs and organoid culture methods. Until the current
limitations of the iPSCs and organoid culture methods will be alleviated, the primary CRC HAE …

… and functionally simulate human airway epithelium to a … organoid model of the human airway
epithelium. The long-term expansion of lung organoids and differentiated airway organoids …

… The apical-out airway organoids morphologically and functionally recapitulated the human
airway epithelium at a comparable level to the apical-in airway organoids. Moreover, apical-…

… organoids from human adult-derived cells has not previously been reported. Here we combined
human adult primary bronchial epithelial … conditions to generate airway organoids. We …

… of well-differentiated human airway epithelial cells, bronchospheres do not … airway epithelial
morphogenesis, as well as a basis for studying the function of the human airway epithelium …