Isolation and culture of human alveolar type II pneumocytes

IR Witherden, TD Tetley - Human airway inflammation: sampling …, 2001 - Springer
… and do not always exhibit classic type II cell characteristics. … human alveolar type II cells.
Some of these methods result in mixed cell populations, which are useful when specific type II …

Human alveolar epithelial type II cells in primary culture

P Mao, S Wu, J Li, W Fu, W He, X Liu… - Physiological …, 2015 - Wiley Online Library
Alveolar epithelial type II ( AEII ) cells are a key … human AEII cells to facilitate the investigation
of the mechanisms of lung diseases at the cellular and molecular levels. Adult human

Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells

JJ Kathiriya, C Wang, M Zhou, A Brumwell… - Nature cell …, 2022 - nature.com
… Loss of alveolar type 2 cells (AEC2s) and the ectopic … Here we demonstrate that human
alveolar type 2 cells (hAEC2s), … into basal cells through alveolar-basal intermediates that …

[HTML][HTML] SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells

EC Mossel, J Wang, S Jeffers, KE Edeen, S Wang… - Virology, 2008 - Elsevier
… is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II
cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS…

Innate immune response of human alveolar type ii cells infected with severe acute respiratory syndrome–coronavirus

Z Qian, EA Travanty, L Oko, K Edeen… - American journal of …, 2013 - atsjournals.org
Alveolar epithelial cells are known to be susceptible in vivo, but the extent of infection for
submerged primary cultures of human type II … that primary human alveolar type II cells can be …

Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-λ1) in response to influenza A infection

J Wang, R Oberley-Deegan, S Wang… - The Journal of …, 2009 - journals.aai.org
… pneumonia, studies on primary human alveolar epithelial cells are limited. The alveolar
epithelium lines the gas … The alveolar epithelium is composed of two main cell types: type I and …

Human alveolar type II cells secrete and absorb liquid in response to local nucleotide signaling

PF Bove, BR Grubb, SF Okada, CMP Ribeiro… - Journal of Biological …, 2010 - ASBMB
… It remains unclear whether the alveolus exhibits an intra-alveolar ion/liquid transport …
isolated human alveolar type II (AT2) cells were utilized to investigate this question. Human AT2 …

Biology of alveolar type II cells

RJ Mason - Respirology, 2006 - Wiley Online Library
… fluid has been well defined in human and animal models. Importantly, beta adrenergic
agonists can stimulate transepithelial sodium transport and facilitate clearance of …

Differentiated Human Alveolar Epithelial Cells and Reversibility of their Phenotype In Vitro

J Wang, K Edeen, R Manzer, Y Chang… - American journal of …, 2007 - atsjournals.org
human alveolar type II cells. Cells cultured in both systems displayed distinct characteristics
of type … First, we cultured freshly isolated human alveolar type II cells on tissue culture plastic …

Innate immune response to influenza A virus in differentiated human alveolar type II cells

J Wang, MP Nikrad, T Phang, B Gao… - American journal of …, 2011 - atsjournals.org
… in differentiated human alveolar Type II cells, and demonstrates that the alveolar epithelium
… Avian influenza is thought to target alveolar epithelial cells because of the type of sialic acid …