Production of human lactoferrin and lysozyme in the milk of transgenic dairy animals: past, present, and future

CA Cooper, EA Maga, JD Murray - Transgenic research, 2015 - Springer
human milk, multiple lines of transgenic dairy animals that produce either human lactoferrin
or human … engineered dairy animals that produce either recombinant lactoferrin or lysozyme …

Production of human lactoferrin in animal milk

IL Goldman, SG Georgieva, YG Gurskiy… - … and Cell Biology, 2012 - cdnsciencepub.com
… The expression constructs of the human lactoferrin (hLf) gene that were used for
producing transgenic animals. Two copies of the insulator are indicated with crosses; the β-casein …

Consumption of transgenic cows' milk containing human lactoferrin results in beneficial changes in the gastrointestinal tract and systemic health of young pigs

CA Cooper, KM Nelson, EA Maga, JD Murray - Transgenic research, 2013 - Springer
… The results from this study show that feeding milk from transgenic cows containing recombinant
human lactoferrin improved GI and immune health and potentially increased the pigs’ …

Expression of human lactoferrin in milk of transgenic mice

GJ Platenburg, EPA Kootwijk, PM Kooiman… - Transgenic …, 1994 - Springer
… A suitable way to meet these criteria is expression via milk of transgenic dairy animals. Since
… hLF expressed in milk of transgenic animals are similar to those of human milk-derived LF. …

Expression of active recombinant human lactoferrin in the milk of transgenic goats

J Zhang, L Li, Y Cai, X Xu, J Chen, Y Wu, H Yu… - Protein Expression and …, 2008 - Elsevier
… The transgenic animals, integrated with the transgene, were then identified with polymerase
… The goats carrying hLf cDNA were mated with nontransgenic animals to produce transgenic

Characterization of recombinant human lactoferrin secreted in milk of transgenic mice

JH Nuijens, PHC van Berkel, MEJ Geerts… - Journal of Biological …, 1997 - ASBMB
Human lactoferrin (hLF) is an iron-binding protein involved in host defense against infection
and severe inflammation. Transgenic mice were produced harboring either hLF cDNA or …

Large scale production of recombinant human lactoferrin in the milk of transgenic cows

PHC Van Berkel, MM Welling, M Geerts… - Nature …, 2002 - nature.com
… report on the generation of transgenic cows expressing recombinant human lactoferrin (rhLF)
in their milk, and on the properties of rhLF as compared with natural hLF from human milk. …

Human lactoferrin transgenic rabbits produced efficiently using dimethylsulfoxide–sperm-mediated gene transfer

L Li, W Shen, L Min, H Dong, Y Sun… - Reproduction, Fertility …, 2006 - CSIRO Publishing
Transgenic animal mammary gland bioreactors are used to produce recombinant proteins.
… the human lactoferrin (LF) gene was cloned and tissue-expressed in the glands of transgenic

The effect of recombinant human lactoferrin from the milk of transgenic cows on Salmonella enterica serovar typhimurium infection in mice

J Wu, Y Hu, C Du, J Piao, L Yang, X Yang - Food & function, 2016 - pubs.rsc.org
… Given this beneficial effect, transgenic approaches have been used to produce lactoferrin. …
effect of recombinant human lactoferrin (rhLF) from the milk of transgenic cows on Salmonella …

Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows

M Wang, Z Sun, T Yu, F Ding, L Li, X Wang, M Fu… - Scientific Reports, 2017 - nature.com
… bioreactor is attributable to the pronuclear microinjection technique used and the long
waiting period required to establish transgenic animal lines 5 . Although our group has recently …