… we used the pH range at which HLA–DM is active (citrate phosphate buffer; pH 5.5). However,
because asparagine can theoretically form hydrogen bonds at a range of higher pH values…

… these hydrogen bonds toward HLA-DM action, we prepared peptide variants that lacked the
ability to form one or more of the hydrogen bonds as … by HLA-DM. We found that disruption of …

… that the conserved hydrogen bond formed by histidine β81 is not a primary target in the DM
catalytic … hydrogen bonds is a critical target necessary for DM-catalyzed peptide dissociation. …

… contribution of hydrogen bonds to DM susceptibility, we tested different DR1:peptide complexes
(all covalently stabilized through a cysteine at P6) for their ability to bind to DM by SPR. …

… DM-mediated prebound peptide release. One likely candidate is the conserved hydrogen
bond (H-bond) … the peptide at position −1 (β81 H-bond). In specific conditions, this particular H-…

… DM specifically targets the H-bond formed by the conserved histidine at position β81 in MHCII
molecules.43 HLA… by DM, possibly because DM cannot disrupt a hydrogen bond that does …

… binding DR1–peptide complexes were converted into efficient HLA-DM binders … hydrogen
bonds to DR1. Peptides that filled the P1 pocket were protected from removal by HLA-DM …

… As in the HLA-DM-HLA-DO structure, αW43 in HLA-DR1 rotates out of the lateral wall of
P1 pocket to directly interact with HLA-DM by forming a hydrogen bond with HLA-DM αN125. …

… of the structure of the HLA-DM molecule showing the α chain (… hydrogen bonds between
the MHC side chains and the backbone of the bound peptide. There are 12–15 hydrogen bonds …

… , we suggest that DM could function by stabilizing a peptide:MHC intermediate in which one
or more hydrogen bonds between the … Editing of the HLA-DR-pep tide repertoire by HLADM. …