Methods to detect hydrogen peroxide in living cells: Possibilities and pitfalls
MB Grisham - Comparative Biochemistry and Physiology Part A …, 2013 - Elsevier
… mediators of cell and tissue damage, others have been shown to be crucial for cell survival
… as a major second messenger in redox signaling is hydrogen peroxide (H 2 O 2 ). This small…
… as a major second messenger in redox signaling is hydrogen peroxide (H 2 O 2 ). This small…
Methods for detection and measurement of hydrogen peroxide inside and outside of cells
… Hydrogen peroxide (H2O2) is an incompletely reduced metabolite of oxygen … living cells
depending on the extent, timing, and location of its production. Characterization of the cellular …
depending on the extent, timing, and location of its production. Characterization of the cellular …
A selective, cell-permeable optical probe for hydrogen peroxide in living cells
MCY Chang, A Pralle, EY Isacoff… - Journal of the American …, 2004 - ACS Publications
… We next assessed the ability of PF1 to operate within living cells. HEK cells were incubated
with 5 μM PF1 for 5 min at 25 C and show negligible intracellular background fluorescence (…
with 5 μM PF1 for 5 min at 25 C and show negligible intracellular background fluorescence (…
A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells
BC Dickinson, CJ Chang - Journal of the American Chemical …, 2008 - ACS Publications
… moiety for detection of hydrogen peroxide localized to cellular mitochondria. Confocal … cell
types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide …
types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide …
Local generation and imaging of hydrogen peroxide in living cells
YA Bogdanova, C Schultz… - Current protocols in …, 2017 - Wiley Online Library
Described here is a localized H 2 O 2 generation‐detection system consisting of a yeast D‐amino
acid oxidase (DAAO) and two spectrally distinct variants of biosensor, HyPer2 and …
acid oxidase (DAAO) and two spectrally distinct variants of biosensor, HyPer2 and …
An ICT-based approach to ratiometric fluorescence imaging of hydrogen peroxide produced in living cells
D Srikun, EW Miller, DW Domaille… - Journal of the American …, 2008 - ACS Publications
… peroxide. Two-photon confocal microscopy experiments in live macrophages show that PL1
can ratiometrically visualize localized hydrogen peroxide bursts generated in living cells at …
can ratiometrically visualize localized hydrogen peroxide bursts generated in living cells at …
Preparation and use of MitoPY1 for imaging hydrogen peroxide in mitochondria of live cells
… of live biological specimens and responds to local fluxes of hydrogen peroxide (H 2 O 2 ) by
… MitoPY1 can be used to measure mitochondrial H 2 O 2 levels in both cell culture and tissue …
… MitoPY1 can be used to measure mitochondrial H 2 O 2 levels in both cell culture and tissue …
Mitochondria-targeted fluorescent probe for imaging hydrogen peroxide in living cells
J Xu, Y Zhang, H Yu, X Gao, S Shao - Analytical chemistry, 2016 - ACS Publications
… targets the mitochondria in living cells and is sensitive to … living cells, and the fluorescence
colocalization studies indicate that the probe localizes solely in the mitochondria of HeLa cells…
colocalization studies indicate that the probe localizes solely in the mitochondria of HeLa cells…
A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells
BC Dickinson, C Huynh, CJ Chang - Journal of the American …, 2010 - ACS Publications
… selectively imaging hydrogen peroxide (H 2 O 2 ) generated at physiological cell signaling
… during the phagocytic respiratory burst and A431 cells in response to EGF stimulation. We …
… during the phagocytic respiratory burst and A431 cells in response to EGF stimulation. We …
Development of a Novel Amplifiable System to Quantify Hydrogen Peroxide in Living Cells
… in live cells and have become indispensable tools in cell … quantify calcium concentrations
in cells. Following the same … ) that can quantify GSH concentrations in live cells, (2) along with …
in cells. Following the same … ) that can quantify GSH concentrations in live cells, (2) along with …
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