… that preconditioning MSCs in hypoxic culture before spheroid … were preconditioned in 1%
O 2 in monolayer culture for 3 days (PC3) or kept in ambient air (PC0), formed into spheroids of …

… in vitro intermittent hypoxic preconditioning combined with 3D spheroid culture can promote
… under hypoxic conditions at appropriate culture concentration and hypoxic conditions. …

… Conclusions Pre-conditioning with hypoxia and HMA … reveal whether pre-conditioning of
spheroids with hypoxia and HMA … However, in spheroid cultures during periods of more than 3 …

… We hypothesize that exposure of MSC spheroids to hypoxic cues or hypoxic-mimicking …
effect of 3D cell culture of MSC spheroids and preconditioning with the hypoxia mimetic DMOG …

… , spheroid cultures precondition hADSCs to a hypoxic environment, and grafting hADSCs
as spheroids to … Spheroid-based cell delivery could be a simple and effective strategy for …

… Therefore, hypoxic preconditioning of MSCs is an effective methodology to optimize MSC …
We have demonstrated that spheroid cultures recapitulate a hypoxic microenvironment that …

… This supports the suggestion that hypoxic preconditioning can prime ASCs to more immediately
drive vascularization upon implantation (16,17,44). In addition, as previously observed, …

… monolayer and spheroid cultures, and spheroid cultures secreted … to be increased in
spheroid cultures in MesenCult-XF … spheroids with diameters >100 μm expressed more hypoxia-…

… the impact of spheroid size on hypoxia-inducible transcription factor (HIF)-1 activity in spheroid
cultures under … Hypoxia could be induced and modulated by controlling the size of the …

… Oxygen measurements in the centre of 3D cell culture constructs were performed as described
previously. In brief, a needle-type oxygen microsensor (PreSens, Regensburg, Germany) …