… suspension bioreactors can be up to 145-fold higher than those in monolayer cultures.
Controlling the size of spheroids can modulate this type of hypoxic preconditioning. …

… In this work, we defined hypoxia as a controlled atmosphere at 1–5% partial pressure of O 2
… Although more studies on the biochemical preconditioning of MSC spheroids cultured under …

… conditions, endothelial cell viability was significantly improved by culture medium
harvested from hypoxic preconditioned ASC (Hyp–ASC-CM) but not by media from non-…

… Mild hypoxic precondition is also established in the inner core of spheroid. This … Our
findings show that formation of spheroids induced mild hypoxic conditions, resulting in the …

… To evaluate the functionality of differently conditioned iMSCs secretomes also in the 3D-culture,
sprouting assays with HUVEC spheroids were performed within collagen/hydroxyapatite …

… preconditioning (HPC), to enhance the retention and survival … hypoxic preconditioning at
0.5% oxygen concentration at different time periods of hypoxia and passage of MSCs in culture. …

… We also found that preconditioned cells displayed stronger … that hypoxia preconditioning,
combined with imaging of oxygenation and cell death, can improve the quality of the spheroid-…

… cellular preconditioning in the 3D systems [5]. Since … , spheroid culture under partial
hypoxic conditions was shown to reduce senescence and cell death [6], [38]. Enhanced hypoxic …

… culture, contribute to spontaneously formed spheroids known as cardiospheres (Csphs).
Following further culture… ) investigate the effect of hypoxic preconditioning on the pro-angiogenic …

… indicate that hypoxic preconditioning of BMSCs in vitro followed by sphere-forming culture is
… cells cultured as spheroids, the core already constitutes a hypoxic microenvironment which …