… indicate that hypoxic preconditioning of BMSCs in vitro followed by sphere-forming culture is
… cells cultured as spheroids, the core already constitutes a hypoxic microenvironment which …

… This study aimed to optimise in vitro MSC culture conditions to explore whether hypoxic
preconditioning of seeded cells promotes pro-angiogenic factors accelerating neoangiogenesis …

… Similar to the 3D culture strategy, hypoxia preconditioning not only increased the survival
of transplanted MSCs, but also activated the expression of pro-survival and angiogenic factors …

… In this work, we demonstrate the utility of our microfluidic platform for studying 3D cell
cultures within a controlled environment. Our platform facilitates precise spatiotemporal oxygen …

… preconditioning could make the cells resistant to the hypoxic condition of the target tissue.
Furthermore, the spheroid culture … culture or spheroid culture was exposed to hypoxia up to 12 …

… Here, we show that hypoxic preconditioning … , 3D cell cultures are becoming increasingly
popular as they offer a more realistic simulation of the cell's environment. 3D cell cultures also …

… not only the hypoxic niche developed in the spheroid core but … , several cell priming/preconditioning
approaches, including … in cell spheroids by controlling the culture parameters. We …

… of hypoxic culture preconditioning can likely be explained by the fact that MSCs exist in
vivo in hypoxic … The spheroid 3D culture creates a microenvironment where inner layers are …

… within the matrix during in vitro preconditioning, respectively, could … mesenchymal stem cells
as spheroids in ischemic hindlimbs … by hypoxic cell preconditioning within spheroid cultures.…

… To evaluate the functionality of differently conditioned iMSCs secretomes also in the 3D-culture,
sprouting assays with HUVEC spheroids were performed within collagen/hydroxyapatite …