… Ex vivo instruction often begins when cells are still in monolayer culture before spheroid …
Additionally, hypoxic preconditioning enhances the survival of spheroids transplanted into …

… We have previously shown that hypoxic preconditioning of … the effects of hypoxic
preconditioning on angiogenic paracrine … tubular epithelial cells in culture. Nephrol Dial …

… pellet cultures was inhibited by preconditioning with hypoxia. … conditions as a result of
hypoxic preconditioning (5% O 2 ); … 3D) collagen hydrogel cultures as well as pellet cultures [40]. …

… fact that cell lines continue to proliferate in spheroid culture, whereas primary MSCs are mostly
… In MSC-spheroids, we found increased expression of hypoxia markers, including HIF-1α …

… Several culture systems have been described from hanging drop … with hypoxia or PHD
inhibitors during spheroid formation (Unpublished observation). Hypoxia pre-conditioning, the …

… mechanisms by which 3D culture promotes higher survival rate … such as preconditioning cells
with hypoxia or cytokines, … -sized spheroids might be more prone to severe hypoxia leading …

… in cancer employed a 3D cell culture model comprising both tumor spheroids and patient-derived …
Similarly, 3D cell culture technology has been used to study the effect of hypoxia in the …

… hypoxic periods of preconditioned Hep-G2 cells. Thus, we cocultured cells for 6 and 7 days
after hypoxic preconditioning … assay (A) and insulin contents of spheroid culture (B). (A) Cell …

… a model in which hypoxia-induced pre-conditioning of RPE- … 2D culture, we were interested
to test if our findings of hypoxia-… alter the hypoxia-induced quiescence profile in spheroids, …

… whether hypoxic preconditioning … spheroids were developed by direct free-floating culture
of explant-derived cells. B. Fluorescence-activated cell sorting of cardiosphere-like spheroids …