… of the spheroid formation protocol. In this study, we examined the spheroid-forming capacity
of … In MSC-spheroids, we found increased expression of hypoxia markers, including HIF-1α …

… efficiency (number of spheroids formed from the number of … In this work, we defined hypoxia
as a controlled atmosphere at … the biochemical preconditioning of MSC spheroids cultured …

… Moreover, cells which invaded the spheroid formed vessel-like structures within 72 hours, in
… in hypoxia (1% O 2 , after preconditioning the cells as routinely done for 24 hours in hypoxia)…

… -forming assay, we observed higher VEGF levels in hypoxic cultures over time, which most
likely contributed directly to enhanced colony formation in hypoxia … levels in hypoxic cultures …

… within the matrix during in vitro preconditioning, respectively, could … mesenchymal stem cells
as spheroids in ischemic hindlimbs … by hypoxic cell preconditioning within spheroid cultures.…

… hypoxia in the presence of potent mediators (all from Tocris except TNFα (Abcam)) of the two
pathways (Table 1). After spheroid formation, the spheroids … preconditioned MSC spheroids …

… strategies such as preconditioning cells with hypoxia or cytokines… -sized spheroids might be
more prone to severe hypoxia leading … After formation of spheroids in MSC 3D , fluorescence …

… term hypoxic conditioning promoted microvessel formation in … technique for facilitating bone
formation and regeneration by … and/or biomaterials or cell spheroids) to produce 3D objects …

… and incubated overnight to allow for spheroid formation. … , our investigation on spheroids
treated with hypoxia and l-… Hypoxic pre-conditioning and hypoxia mimetic agents support …

… Interestingly, inflammatory mediators added to hypoxia exerted a significant inhibitory effect
on the capacity of total CM and related secretome fractions to induce the formation of tube-…