… were obtained as described above for Western blot analysis. The intensity of fluorescence
of the protein extract was determined using an LS 50B luminescence spectrometer (Perkin-…

… For Western blot analysis, approximately 20 μg of proteins from the cell lysates or 5 to 10
μl of concentrated culture supernatant of A. fumigatus was loaded onto each lane of a sodium …

… Several booster injections were performed every 2 weeks after the first injection, and the
immunization was followed by immunoblot analysis with peroxidase-conjugated anti-mouse …

The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified
by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the …

… applied the immunoblot technique to the serological response in systemic aspergillosis
to … Preparation of Aspergillus fumigatus antigens and their analysis by two dimensional …

… ′, Aspergillus fumigatus; lanes D and D′, Aspergillus giganteus. Note that the Aspergillus
fumigatus … C′ and D′ show an immunoblot with the mitogillin-specific monoclonal antibody …

… fumigatus cell wall were recognised on 2-D immunoblot using sera of patients suffering from
aspergillosis… Aspergillus fumigatus, a human isolate was used throughout the present study …

… Aspergillus fumigatus is a saprotrophic filamentous fungus and … fumigatus ranges from
allergies in immunocompetent hosts to … fumigatus by immunoblot analysis of 2D-electrophoresis …

… Strong immunoreactivity was observed in the low pH range region of the immunoblots at …
correspond with the strong signals on the immunoblot, we presume that the immunostaining in …

… The Western blot assay in which the immunoreactivity of the recombinant form of the
enzyme has been demonstrated is not, however, a user-friendly format for performing routine …