Antigens of Aspergillus fumigatus produced in vivo

J Sarfati, DG Boucias, JP Latgé - Journal of medical and …, 1995 - academic.oup.com
Immunoblots of extracts from kidneys of mice infected intravenously with Aspergillus
fumigatus were probed with human sera from patients with aspergilloma. A limited number of …

A new IgE Western blot identifies Aspergillus fumigatus sensitization and may discriminate allergic bronchopulmonary aspergillosis.

RP Piarroux, JC Dubus, M Reynaud‐Gaubert… - Allergy, 2019 - search.ebscohost.com
western blot (WB) would enable one‐step characterization of sensitization to a greater number
of Af molecules. We aimed to design a WB assay … sensitization to Aspergillus fumigatus in …

Production and characterization of monoclonal antibodies to a 58-kilodalton antigen of Aspergillus fumigatus

PM Fratamico, WK Long, HR Buckley - Infection and immunity, 1991 - Am Soc Microbiol
… study were studied by immunoblot analysis by using ME. … fumigatus and other fungal
antigens have been shown to cross-react in serological assays (15, 19). By immunoblot analysis

cDNA cloning and immunological characterization of a newly identified enolase allergen from Penicillium citrinum and Aspergillus fumigatus

HY Lai, MF Tam, RB Tang, H Chou… - International archives of …, 2002 - karger.com
… 2D immunoblot analysis of components in A. fumigatus CCRC33476 extracts reacted with
human IgE antibodies. a Coomassie blue-stained 2D protein profile of the crude extracts on a …

Expression of the Cu,Zn superoxide dismutase of Aspergillus fumigatus as determined by immunochemistry and immunoelectron microscopy

AJ Hamilton, MD Holdom… - FEMS Immunology & …, 1996 - academic.oup.com
fumigatus SOD which we have used to assess these questions. … Western blot (immunoblot)
and Coomassie brilliant blue stain analysis of reactivity of sheep anti-A. fimigatus …

Molecular cloning of the cDNA and gene for an elastinolytic aspartic proteinase from Aspergillus fumigatus and evidence of its secretion by the fungus during invasion …

JD Lee, PE Kolattukudy - Infection and immunity, 1995 - Am Soc Microbiol
Western blot analysis of the fractions showed that the elution of the enzymatic activity was
Western blot analysis of the unfractionated culture medium showed only one strongly cross-…

Characterization of a cell-wall acid phosphatase (PhoAp) in Aspergillus fumigatus

M Bernard, I Mouyna, G Dubreucq… - …, 2002 - microbiologyresearch.org
… For Western-blot analysis, proteins were blotted onto a nitrocellulose membrane. The
membrane was blocked with 5% defatted milk powder (Regilait) in TBS containing 0·05% Tween …

Humoral and cell-mediated autoimmunity in allergy to Aspergillus fumigatus.

R Crameri, A Faith, S Hemmann, R Jaussi… - The Journal of …, 1996 - rupress.org
fumigatus to recombinant human and A. fumigatus MnSOD was analyzed by an antigen-…
For IgE immunoblot analysis, proteins were separated on an SDS-polyacrylamide gradient …

AFMP2 Encodes a Novel Immunogenic Protein of the Antigenic Mannoprotein Superfamily in Aspergillus fumigatus

KTK Chong, PCY Woo, SKP Lau… - Journal of clinical …, 2004 - Am Soc Microbiol
Western blot analysis showed that recombinant Afmp2p reacted specifically with sera from
patients with aspergilloma or invasive aspergillosis (Fig. 4, lanes 1 to 4). No specific reaction …

The 18-kilodalton antigen secreted by Aspergillus fumigatus

JP Latgé, M Moutaouakil, JP Debeaupuis… - Infection and …, 1991 - Am Soc Microbiol
fumigatus antibodies by immunoblot analysis in one serum sample of six healthy individuals
studied using a 1:100 dilution of serum. In patients with aspergilloma, the increase in theIgG …