… Protein A, a cell membrane protein with a molecular weight … In this present study, the
immunogold nanoparticle (IgG–AuNP) … The quantitative and qualitative detection of protein A was …

… of the antibody to the Protein A/G on the AuNP surface. These results highlight the ideal
immuno-gold nanoparticle characteristics for the detection of target antigens such as cocaine. …

… Next, upon centrifugation at 13,000 rpm for 1 h, two layers were usually obtained: a clear
supernatant of unbound antibody and loosely packed sediment of the immunogold (dark red). …

… is helpful to decrease the cost especially for massive production because excessive antibodies
are unused and are discarded by centrifugation during the preparation of immunogold. …

… preparation of anti-protein A IgG antibodies using labels of gold nanoparticles and the
development … In the absence of protein A (PBS), no immunogold was bound to the solid-phase test …

… In addition, protein A was utilized as a target for the detection of … -protein A IgG antibodies
using labels of gold nanoparticles and … In the absence of protein A (PBS), no immunogold was …

… We propose a new immunoassay entitled ‘in-situ immuno-gold nanoparticle network ELISA’
… for bacteria detection. To improve its analytical performance in direct detection of pathogens …

… In the present study, a simple and effective mark method of antibody to colloidal gold
nanoparticles was developed and evaluated. The rationale of immobilization of Antibody onto …

… gold–protein A conjugates. We also describe a novel sol particle–trypsin assay using conjugates
of gold nanoparticles with … The numerous applications for immunogold conjugates were …

… The number of Abs immobilized onto AuNPs was determined using a modified Bradford
assay (Bio-Rad protein assay). Standard solutions of anti-HRP Ab (0–50 μg/mL) were prepared …