… Integrin endocytic recycling is critical … integrins assemble into new adhesions is unclear. By
synchronizing endocytic disassembly of focal adhesions (FAs), we find that recycled integrins …

… required to establish a stable nascent integrin cluster. Therefore, we hypothesized that
increased integrin activation would overcome this ‘nanolimit’ for stable integrin recruitment. …

… in adhesion, integrin activation can control the polarity of migrating cells and the assembly
of … Blockade of integrin activation may therefore be useful in anti-adhesive therapies 3 . The …

… In order to distinguish between β-1 and β-3 integrins, we examine the effect of varying
integrin activation rates, motility, ligand binding affinity, clustering, and bond kinetics (Fig 2A–2C). …

… adhesion and migration, and extracellular matrix assembly. A final step in integrin activation
is the binding of talin to integrin β … order integrin activation pathways of a prototypic integrin, …

… that FN assembly proceeds through at least two different stages and each stage has
different requirements for integrin activation state and FN sequences. Initially, integrins bind …

… We report that ∼100-nm clusters of ∼50 β3-activated integrins form very early adhesions
… model of adhesion assembly on rigid glass in which the first step involves integrin clustering …

… To test the hypothesis that the activation state of Fn-binding integrins regulates … -binding
integrin are essential elements in assembly of a Fn matrix. These are activation of the integrin to …

… Integrin activation has been classically measured with the multivalent ligand-mimetic IgM
antibody PAC1, suggesting that integrin activation reflected also integrin clustering in addition …

… However, the contributions of integrin clustering and focal adhesion assembly were … adhesion
assembly on cell adhesion strength, it does not discount a central role for focal adhesions …