Analysis of latex protein content by liquid chromatography coupled with tandem mass spectrometry (HPLC/MS/MS)

B Witkowski, A Szabłowska, M Malesa… - Analytical …, 2015 - pubs.rsc.org
… ) latex proteins, but may be inefficient for total protein content evaluation or for correlating …
/MS – based protein quantification protocol for raw latex stabilized with ammonia and latex

Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LCMS/MS

L Dai, G Kang, Z Nie, Y Li, R Zeng - Journal of proteomics, 2016 - Elsevier
… separation step was used prior to LCMS/MS analysis. … , latex proteins of H. brasiliensis
treated with ET and MeJA were analyzed using an iTRAQ-coupled two-dimensional LCMS/MS

Proteomic comparison of Chelidonium majus L. latex in different phases of plant development

R Nawrot, R Lippmann, A Matros, O Musidlak… - Plant Physiology and …, 2017 - Elsevier
… majus latex contains proteins connected with response to stress conditions and generation
… Notable proteins characteristic to latex include major latex protein (MLP, presumably …

Identification of avocado (P ersea americana) pulp proteins by nano‐LCMS/MS via combinatorial peptide ligand libraries

C Esteve, A D'Amato, ML Marina, MC García… - …, 2012 - Wiley Online Library
protein has not been previously described as an allergen in avocado, it is present in latex
cross-reactivity in cases of latex-fruit syndrome 37. We have also identified a thaumatin-like …

[PDF][PDF] Comparative analysis of protein profiles of toxic and non-toxic Jatropha curcas latex

L Gruneck, E Gentekaki, J Jaresitthikunchai… - … MAI JOURNAL OF …, 2017 - thaiscience.info
… The LCMS raw data was converted in the appropriate format and the PepDetect module of
the DeCyder software was used for automated peptide detection, charge state assignments, …

Comprehensive proteome analysis of lettuce latex using multidimensional protein-identification technology

WK Cho, XY Chen, NM Uddin, Y Rim, J Moon, JH Jung… - Phytochemistry, 2009 - Elsevier
… roles of this latex in higher plants, we embarked on its proteomic analysis. We investigated
587 latex proteins that were identified from the lettuce latex using multidimensional protein-…

Comparison between liquid chromatography/tandem mass spectroscopy and a novel latex agglutination method for measurement of hepcidin-25 concentrations in …

D Kamei, M Nagano, T Takagaki, T Sakamoto… - Heliyon, 2023 - cell.com
… the latex immunoassay (LIA) method using this reagent versus the conventional LC-MS/MS
were sensitized to polystyrene latex and blocked with an inactivated protein to prepare a …

Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward

MAH Habib, MN Ismail - Journal of plant research, 2021 - Springer
… spectrometry (MS). In this manuscript, we reviewed the methods of latex protein extraction
that … decades as well as the results of numerous latex protein identification and quantitation. …

Structural analysis of the glycoprotein allergen Hev b 4 from natural rubber latex by mass spectrometry

D Kolarich, F Altmann, E Sunderasan - Biochimica et Biophysica Acta (BBA …, 2006 - Elsevier
… of metabolites and proteins. There is a consensus that certain proteins or peptides in the latex
products, … A small proportion of these proteins is responsible for NRL allergy, which is an …

Label-free quantitative proteomics reveals differentially regulated proteins in the latex of sticky diseased Carica papaya L. plants

SP Rodrigues, JA Ventura, C Aguilar, ES Nakayasu… - Journal of …, 2012 - Elsevier
… ], and analyzed by 2D-LC-ESI-MS/MS. However, just a … latex proteins in the presence of
100 μM E-64, ten times more than usually recommended, and immediately separate the proteins